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233153 Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Dithiothreitol, DTT

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233153
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Descripción

Replacement Information

Tabla espec. clave

CAS #Empirical Formula
3483-12-3C₄H₁₀O₂S₂

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
233153-10GM
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      233153-1GM
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          233153-5GM
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              Description
              OverviewA protective agent for SH groups.
              Absorbance: (100 mM, 280 nm):≤0.1. Oxidized dithiothreitol:≤0.5%.
              Catalogue Number233153
              Brand Family Calbiochem®
              SynonymsDithiothreitol, DTT
              References
              ReferencesChau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
              Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
              Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
              Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
              Shaked, Z., et al. 1980. Biochemistry 19, 4156.
              Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
              Cleland, W.W. 1964. Biochemistry 3, 480.
              Product Information
              CAS number3483-12-3
              FormWhite solid
              Hill FormulaC₄H₁₀O₂S₂
              Chemical formulaC₄H₁₀O₂S₂
              Quality LevelMQ100
              Applications
              Biological Information
              Purity≥99% by titration
              Physicochemical Information
              ContaminantsHeavy metals: <1 ppm
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSEK1610000
              Safety Information
              R PhraseR: 22-36/38

              Harmful if swallowed.
              Irritating to eyes and skin.
              S PhraseS: 26-36

              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Wear suitable protective clothing.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
              Packaging Information
              Packaged under inert gas Packaged under inert gas
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Número de referencia GTIN
              233153-10GM 04055977217650
              233153-1GM 04055977217643
              233153-5GM 07790788054267

              Documentation

              Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem Ficha datos de seguridad (MSDS)

              Título

              Ficha técnica de seguridad del material (MSDS) 

              Cleland’s Reagent, ULTROL® Grade - CAS 3483-12-3 - Calbiochem Certificados de análisis

              CargoNúmero de lote
              233153

              Referencias bibliográficas

              Visión general referencias
              Chau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
              Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
              Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
              Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
              Shaked, Z., et al. 1980. Biochemistry 19, 4156.
              Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
              Cleland, W.W. 1964. Biochemistry 3, 480.
              Ficha técnica

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision27-August-2018 JSW
              SynonymsDithiothreitol, DTT
              DescriptionDTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes, protecting protein sulfhydryls and restoring enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.
              FormWhite solid
              Intert gas (Yes/No) Packaged under inert gas
              CAS number3483-12-3
              RTECSEK1610000
              Chemical formulaC₄H₁₀O₂S₂
              Purity≥99% by titration
              ContaminantsHeavy metals: <1 ppm
              SolubilityEthanol or H₂O (200 mM)
              Storage +2°C to +8°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.
              Toxicity Harmful
              Merck USA index14, 3376
              ReferencesChau, M-H., and Nelson, J.W. 1991. FEBS Lett. 291, 296.
              Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
              Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
              Zhang, R., and Snyder, G.H. 1988. Biochemistry 27, 3785.
              Shaked, Z., et al. 1980. Biochemistry 19, 4156.
              Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
              Cleland, W.W. 1964. Biochemistry 3, 480.