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HTS005M ChemiSCREEN™ CCR1 Membrane Preparation

Human CCR1 GPCR membrane preparation for Radioligand binding Assays.

ChemiSCREEN™ CCR1 Membrane Preparation: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Host Cell: Chem-2 Uni Prot Number: P32246 Entrez Gene Number: NM_001295.2
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HTS005M
1 mL  
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      Descripción

      Replacement Information

      Tabla espec. clave

      SpeciesHost CellsProtein Target
      Human Chem-2CCR1
      Description
      Catalogue NumberHTS005M
      Brand Family Chemicon®
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™ CCR1 Membrane Preparation
      OverviewFull-length human CCR1 cDNA
      Background InformationCCR1 is a GPCR that binds to a variety of CC ligands, including MIP-1a, RANTES, MCP-3, HCC-1, HCC-2, HCC-4, and MPIF-1 (Olson and Ley, 2002). Lymphocytes, macrophages, dendritic cells, and GM-CSF-activated neutrophils express CCR1 (Kaufmann et al., 2001; Cheng et al., 2001). Two selective, non-peptide small molecule antagonists of CCR1, BX-471 and CP-481,715, have been synthesized (Gladue et al., 2003; Liang et al., 2000). Pharmacological and genetic targeting of CCR1, either alone or in combination with cyclosporin A, reduces cardiac and renal allograft rejection (Gao et al., 2000; Horuk et al., 2001a; Horuk et al., 2001b), allergic encephalomyelitis (Liang et al., 2000), and renal fibrosis (Anders et al., 2002) in experimental models. Chemicon's CCR1 Membrane Preparations are ideal for screening molecules that disrupt interaction between CCR1 and its ligands. The CCR1 Membrane Preps bind to MIP-1a with a Kd of 1.8 nM, and yield greater than 10-fold signal to background ratio with a MIP-1a concentration of 0.1 nM.
      References
      Product Information
      FormatMembranes
      PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
      Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
      Each vial typically contains 500 units, where one unit is the amount of membrane prep that yields greater than 1000 cpm specific binding of 125I-labeled MIP-1α (0.1 nM input concentration).
      Quality LevelMQ100
      Applications
      ApplicationHuman CCR1 GPCR membrane preparation for Radioligand binding Assays.
      Key Applications
      • Radioligand binding assay
      Application NotesRadioligand binding assay.
      Biological Information
      Concentration0.5 mg/mL
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p.
      Gene Symbol
      • CCR1
      • HM145
      • CCR-1
      • MIP1aR
      • RANTES-R
      • CKR-1
      • CD191
      • CMKBR1
      • CC-CKR-1
      • MIP-1alpha-R
      • SCYAR1
      • CMKR1
      GPCR ClassA
      Host CellsChem-2
      Protein TargetCCR1
      Target Sub-FamilyChemokine
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P32246 # Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1- beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.
      SIZE: 355 amino acids; 41173 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Widely expressed in different hematopoietic cells.
      SIMILARITY: SwissProt: P32246 ## Belongs to the G-protein coupled receptor 1 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceSPECIFICATIONS:
      Bmax for [125I]MIP-1alpha binding: 3.3 pmol/mg protein
      Kd for [125I] MIP-1alpha binding: ~ 1.8 nM HOST CELLS:
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      HTS005M 04053252619908

      Documentation

      Protocols

      Title
      Assembly of a Competition Binding Curve

      ChemiSCREEN™ CCR1 Membrane Preparation Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      ChemiSCREEN™ CCR1 Membrane Preparation Certificados de análisis

      CargoNúmero de lote
      ChemiscreenTM MEMBRANE PREPARATION HUMAN RECOMBINANT CCR1 CHEMOKINE RECEPTOR - 2349735 2349735
      ChemiscreenTM MEMBRANE PREPARATION HUMAN RECOMBINANT CCR1 CHEMOKINE RECEPTOR - 2052325 2052325
      ChemiscreenTM MEMBRANE PREPARATION HUMAN RECOMBINANT CCR1 CHEMOKINE RECEPTOR - 2123198 2123198

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Granulocyte-macrophage colony stimulating factor up-regulates CCR1 in human neutrophils.
      Cheng, S S, et al.
      J. Immunol., 166: 1178-84 (2001)  2001

      Mostrar resumen       		11145699 11145699

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      Categorías

      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
      Life Science Research > Proteins and Enzymes > GPCR Membrane Preparations