Millipore Sigma Vibrant Logo

219399 Cathepsin D Substrate I - Calbiochem

Useful as a substrate for the determination of cathepsin D activity.

Cathepsin D Substrate I - Calbiochem: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Bz-RGFFP-4-MeOβNA, HCl

View Products on Sigmaaldrich.com
219399
Ver precios y disponibilidad

Descripción

Replacement Information

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
219399-10MG
Comprobando disponibilidad...
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      		 Ampolla de plást. 10 mg
      Recuperando precio...
      No pudo obtenerse el precio
      La cantidad mínima tiene que ser múltiplo de
      Maximum Quantity is
      Al finalizar el pedido Más información
      Ahorró ()
       
      —
      Solicitar precio
      		 —
      Description
      OverviewUseful as a substrate for the determination of cathepsin D activity. Suitable for either colorimetric or fluorometric assay. Cathepsin D hydrolyzes the Phe-Phe bond. Requires dipeptidyl aminopeptidase II to free 4-methoxy-β-naphthylamine (4MeOβNA).
      Catalogue Number219399
      Brand Family Calbiochem®
      SynonymsBz-RGFFP-4-MeOβNA, HCl
      References
      ReferencesTakahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
      Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.
      Product Information
      ATP CompetitiveN
      FormWhite lyophilized solid
      Hill FormulaC₄₉H₅₅N₉O₇ · HCl
      Chemical formulaC₄₉H₅₅N₉O₇ · HCl
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetSubstrate for the determination of cathepsin D activity
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceBz-Arg-Gly-Phe-Phe-Pro-4MeOβNA, HCl
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      219399-10MG 04055977201697

      Documentation

      Cathepsin D Substrate I - Calbiochem Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Cathepsin D Substrate I - Calbiochem Certificados de análisis

      CargoNúmero de lote
      219399

      Referencias bibliográficas

      Visión general referencias
      Takahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
      Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.

      Folleto

      Cargo
      Cathepsins and Related Products Technical Bulletin
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-June-2008 RFH
      SynonymsBz-RGFFP-4-MeOβNA, HCl
      DescriptionA useful substrate for colorimetric or fluorometric assay of cathepsin D activity. Cathepsin D hydrolyzes the Phe-Phe bond. Dipeptidyl aminopeptidase II is required to free the 4-methoxy-β-naphthylamine (4MβNA). Excitation max: ~345 nm; emission max: ~425 nm.
      FormWhite lyophilized solid
      Recommended reaction conditions
      1. Incubate the substrate, Bz-Arg-Gly-Phe-Phe-Pro-4MβNA (final concentration 350 µM) with cathepsin D in 500 µl of reaction buffer (20 mM glycine-HCl, pH 3.0) at 37°C to allow digestion of the substrate to Bz-Arg-Gly-Phe-OH and Phe-Pro-4MβNA. 2. Stop the reaction by adding 50 µl of 1% KOH and then add 850 µL of reaction buffer 2 (100 mM Tris-HCl, pH 7.8). 3. Add 1 mU dipeptidylpeptidase IV in 100 µl of reaction buffer 2 and incubate for >1 h at 37°C to completely hydrolyze the Phe-Pro-4MβNA to Phe-Pro-OH and 4MβNA. 4. Monitor the release of 4MβNA at 345 nm (excitation) and 425 nm (emission).
      Chemical formulaC₄₉H₅₅N₉O₇ · HCl
      Peptide SequenceBz-Arg-Gly-Phe-Phe-Pro-4MeOβNA, HCl
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage Protect from moisture
      Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Toxicity Standard Handling
      ReferencesTakahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
      Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.