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71285 BCA Protein Assay Kit

The BCA protein assay is a simple and reliable protein quantification method.

BCA Protein Assay Kit: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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71285
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Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
71285-3
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      Description
      OverviewThe BCA protein assay is based on a biuret reaction, which is the reduction of Cu2+ to Cu+ by proteins in an alkaline solution with concentration-dependent detection of the monovalent copper ions. Bicinchoninic acid is a chromogenic reagent that chelates the reduced copper, producing a purple complex with strong absorbance at 562 nm (Smith 1985, Wiechelman 1988). This assay can be used to quantify protein concentration with a wide variety of samples and can be performed in minutes.

      The Novagen® BCA Protein Assay Kit can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration. Kit components are sufficient to complete 500 standard-size reactions (50 µl protein sample plus 1 ml reagent) or 2500 micro-scale reactions (25 µl protein sample plus 200 µl reagent). A BSA standard (2 mg/ml) is provided for convenient and reliable preparation of standard curves.

      This assay is robust and can be performed in the presence of many compounds. Some reagents, including chelating agents, strong acids or bases, and reducing agents, interfere with the reduction and chelating reactions on which this assay depends (Brown 1989). The BCA assay is compatible with the following Novagen protein extraction and lysis reagents: BugBuster® Protein Extraction Reagent, PopCulture® Reagent, CytoBuster™ Protein Extraction Reagent, Reportasol™ Extraction Buffer, and Insect PopCulture Reagent. Options for the removal or dilution of interfering substances are described in the kit literature.
      Catalogue Number71285
      Brand Family Novagen®
      References
      ReferencesSmith, P.K., et al. 1985. Anal. Biochem. 150, 76.

      Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.

      Wiechelman, K., et al. 1988. Anal. Biochem. 175, 231.

      Brown, R., et al. 1989. Anal. Biochem. 180, 136.
      Product Information
      •500 mlBCA Solution
      •15 ml4% Cupric Sulfate
      •3 x 1 mlBSA Standard, 2 mg/ml
      DeclarationUse of the BCA protein assay is permitted for research purposes only.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      71285-3 07790788060435

      Documentation

      Licencias necesarias

      Título
      CARTA DE USO GENERAL 2016
      PRODUCTO REGULADO POR LA SAGARPA
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      BCA Protein Assay Kit Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      BCA Protein Assay Kit Certificados de análisis

      CargoNúmero de lote
      71285

      Referencias bibliográficas

      Visión general referencias
      Smith, P.K., et al. 1985. Anal. Biochem. 150, 76.

      Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.

      Wiechelman, K., et al. 1988. Anal. Biochem. 175, 231.

      Brown, R., et al. 1989. Anal. Biochem. 180, 136.

      Citas

      Título
    • Matthias E. Liechti, et al. (2007) Metabotropic glutamate 2/3 receptors in the ventral tegmental area and the nucleus accumbens shell are involved in behaviors relating to nicotine dependence. Journal of Neuroscience 27, 9077-9085.
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      Protocolos de usuario

      Cargo
      TB380 BCA Protein Assay Kit