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04-281 Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.

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Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: EGFR, ERBB1, ERBB, mENA UniProt Number: P00533
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04-281
100 µg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-281
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Thr669) Antibody, clone 5F10
      Alternate Names
      • ERBB
      • ERBB1
      • epidermal growth factor receptor
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Includes PMA and pervanadate treated A431 cell lysate as a positive control
      Presentation100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.
      Clone5F10
      HostMouse
      SpecificityRecognizes EGFR when phosphorylated at Threonine 669.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Modifications
      • Phosphorylation
      Purification MethodThiophilic adsoption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions 6 months at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Número de referencia GTIN
      04-281 04053252744242