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MAB4092 Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: SELPLG, CLA, PSGL-1, CD162, PSGL1 UniProt Number: Q14242
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MAB4092
100 µg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IHC, IH(P), IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4092
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1
      Alternate Names
      • PSGL-1
      • CD162
      Background InformationInteractions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.
      References
      Product Information
      FormatPurified
      PresentationPurified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000

      FACS {Snapp, KR et al 1998}.

      Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).

      Functional blocking of P-selectin {Snapp KR et al 1998}.

      Immunohistochemistry: Fresh frozen, acetone fixed sections

      Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenThe antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).
      CloneKPL-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityP-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteKnown not to react with Rat, Bovine, Pig, and Equine PSGL-1 {Snapp, KR et al 1998; Baisse, B et al 2007}.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.
      Gene Symbol
      • SELPLG
      • CLA
      • PSGL-1
      • CD162
      • PSGL1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14242 # Binds to P-, E- and L-selectins. The calcium-dependent high affinity interaction with P-selectin mediates the tethering and rolling of neutrophils and T-lymphocytes on endothelial cells.
      SIZE: 412 amino acids; 43201 Da
      SUBUNIT: Homodimer; disulfide-linked.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes.
      PTM: Displays complex, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Also N- glycosylated. & Sulfated in the N-terminal region; sulfation is necessary for P-selectin binding.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MAB4092 04053252667763

      Documentation

      Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 Certificados de análisis

      CargoNúmero de lote
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY - 2383971 2383971
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY - 2359959 2359959
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3231554 3231554
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3680716 3680716
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3946452 3946452
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3959180 3959180
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 4135337 4135337
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2594838 2594838
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2614679 2614679
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2635046 2635046
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      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions.
      Long, M, et al.
      Annals of biomedical engineering, 29: 935-46 (2001)  2001

      Mostrar resumen       		Inhibits Activity/Function11791676 11791676

      Ficha técnica

      Cargo
      MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY