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06-680 Anti-Androgen Receptor Antibody

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06-680
100 µL  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IP, FC, ChIP, IHC, ChIP-seqRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-680
      DescriptionAnti-Androgen Receptor Antibody
      Alternate Names
      • Androgen receptor
      • Dihydrotestosterone receptor
      • Nuclear receptor subfamily 3 group C member 4
      Background InformationAndrogen receptor (UniProt: P15207; also known as Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR) is encoded by the Ar (also known as Nr3c4) gene (Gene ID: 24208) in rat. AR is a steroid hormone receptor that mediates the action of testosterone and dihydrotestosterone. AR is shown to be predominantly cytoplasmic in its unligated form, but translocates to the nucleus upon ligand-binding. It can also translocate to the nucleus in unligated form in the presence of RACK1. The AR contains three major functional domains - the N-terminal transcriptional regulation domain, the DNA binding domain (DBD; aa 541-614) and the ligand binding domain (aa 651-882). The DBD contains two C4-type zinc fingers (aa 542-562 and 578-602) that recognize specific DNA consensus sequences and facilitate direct DNA binding of the AR to the promoter and enhancer regions of AR-regulated genes. AR is shown to be phosphorylated in prostate cancer cells in response to several growth factors including EGF. This phosphorylation is induced by c-Src kinase. In AR, tyrosine 517 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity are associated with prostate cancer progression. Phosphorylation of AR by Tyrosine kinase non receptor 2 (TNK2) is reported to enhance the DNA-binding and transcriptional activity. AR can also be phosphorylated at serine 61 by Cdk9, which regulates its promoter selectivity and cell growth. (Ref.: Davey, RA., and Grossmann, M. (2016). Clin. Biochem. Rev. 37(1); 3-15).
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Androgen Receptor, PG-21, Cat. No. 06-680, is a rabbit polyclonal antibody that detects Androgen receptor and is tested in ChIP-seq, ChIP, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Flow Cytometry
      • Chromatin Immunoprecipitation (ChIP)
      • Immunohistochemistry
      • ChIP-seq
      Application NotesChromatin Immunoprecipitation (ChIP) Analysis: 1 µg from a representative lot detected Androgen Receptor in Sonicated chromatin prepared from hormone starved (72H), then induced (1 µM CI-4AS-1) overnight LNCaP cells (~1e5 cell equivalents per IP).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in MCF-7 cells stabily transfected with Androgen Receptor (Ghatge, R. P., et al. (2005). Breast Cancer Res. 7(6): R1036-1050).

      Immunohistochemistry Applications: A representative lot from an independent laboratory detected Androgen Receptor in embryonic rat tissue, neonatal rat testis tissue, and adult rat testis tissue (Nation, T., et al. (2010). J Pediatr Surg. 46(8): 1539-1543.).

      ChIP-seq: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from LNCaP cell lysates under certain treated conditions (Zhao, J. C., et al. (2012). Genome Res. 22(2):322-331.).

      Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Androgen Receptor in LNCap cell lysate.

      Flow Cytometry Analysis: 1 µg/mL from a representative lot detected Androgen Receptor in MCF-7 cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the first 21 amino acids from the N-terminal region of rat Androgen Receptor.
      EpitopeThis antibody recognizes the Modulation Region within the N-terminus of Androgen Receptor.
      HostRabbit
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman. Predicted to react with Bovine, Canine, Horse, Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ar
      • Nr3c4
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~110 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in LnCap cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Androgen Receptor in LnCap cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Número de referencia GTIN
      06-680 08436037123856