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70933 Sigma-AldrichpTriEx™-4 Neo DNA - Novagen

pTriEx™-4 Neo DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
User Protocol
Citations
Vector Map
Vector Sequence

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Overview

Replacement Information

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Description
Overview	The pTriEx™-4 Neo vector is designed to allow rapid characterization of target genes in E. coli, insect and vertebrate cells, and to allow rapid selection of stable transfected vertebrate cells expressing high levels of target gene. Expression in vertebrate cells is mediated by the CMV immediate early enhancer and promoter². The drug selection marker is expressed under the control of the EMC virus Cap-Independent Translation Enhancer (CITE) sequence (or IRES), allowing rapid selection of transfected vertebrate cells using the drug G418 or neomycin sulfate. For expression in insect cells, pTriEx-4 Neo contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector^® System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λlp_L and λp_I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows induction with IPTG. Native protein can be expressed by cloning into the Nco I site, or generated by cloning into the PshA I or Sma I sites and cleaving the fusion protein with enterokinase or thrombin, respectively.
Overview	This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number	70933
Brand Family	Novagen®

References

Product Information
Quality Level	MQ100

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped with Blue Ice or with Dry Ice
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
70933-3	07790788060961

Documentation

pTriEx™-4 Neo DNA - Novagen SDS

Title
Safety Data Sheet (SDS)

pTriEx™-4 Neo DNA - Novagen Certificates of Analysis

Title	Lot Number
70933	Enter Lot Number

Citations

Title

Saurabh K. Gupta, et al. (2007) Human T-cell leukemia virus type1 tax oncoprotein prevents DNA damage-induced chromatin egress of hyperphosphorylated huChk2. Journal of Biological Chemistry 282, 29431-29440.

Gerard M. Gibbs, et al. (2006) The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. Journal of Biological Chemistry 281, 4156-4163.

Bratislav Janjic, et al. (2006) Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. Journal of Immunology 177, 2717-2727.

Abdelhakim Ahmed-Belkacem, et al. (2005) Flavonoid structure-activity studies identify 6-prenylchrysin and tectochrysin as potent and specific inhibitors of breast cancer resistance protein ABCG2. Cancer Research 65, 4852-4860.

User Protocols

Title
TB053 Academic and Non-profit Laboratory Assurance Letter
TB250 pTriEx™ System Manual

Vector Map

Title
TB299 pTriEx-4 Neo Vector Map

Vector Sequence

Title
pTriEx-4 Neo Sequence

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