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14-404 ZAP-70 Protein, active, 20 µg

Active, recombinant, human, full length ZAP-70 containing a C-terminal His6 tag, for use in Kinase Assays.

ZAP-70 Protein, active, 20 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-404
20 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-404
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionZAP-70 Protein, active, 20 µg
      OverviewRecombinant, human, full length ZAP-70 containing a C-terminal His6 tag
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, recombinant, human, full length ZAP-70 containing a C-terminal His6 tag, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in Sf21
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
      Gene Symbol
      • ZAP70
      • ZAP-70
      • STD
      • TZK
      • SRK
      Protein TargetZAP-70
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
      SIZE: 619 amino acids; 69872 Da
      SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
      TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
      DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
      PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
      DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
      Molecular Weight70 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1) substrate peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -20C
      Packaging Information
      Material Size20 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-404 04053252581793

      Documentation

      ZAP-70 Protein, active, 20 µg SDS

      Title

      Safety Data Sheet (SDS) 

      ZAP-70 Protein, active, 20 µg Certificates of Analysis

      TitleLot Number
      ZAP-70, active - 25510U-B 25510U-B
      ZAP-70, active - D9CN010U-A D9CN010U-A

      References

      Reference overviewPub Med ID
      The Syk family of protein tyrosine kinases in T-cell activation and development.
      Chu, D H, et al.
      Immunol. Rev., 165: 167-80 (1998)  1998

      		9850860 9850860

      FAQ

      QuestionAnswer
      Some of my enzyme preparations require Brij-35, what is its purpose?The role of Brij-35 is to function as a mild detergent, which prevents aggregation in active kinases.