Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
25-September-2014 JSW
Description
Native, bovine thrombin. Key enzyme in the coagulation cascade. Converts fibrinogen into fibrin and activates factor XIII, which cross-links and stabilizes the fibrin polymer. Has a pH of 6.0-7.5 in a 7% solution.
Form
Lyophilized
Formulation
Lyophilized from 50 mM Sodium Citrate, 0.2 M NaCl, 0.1% PEG-8000, pH 6.5.
Concentration Label
Please refer to vial label for lot-specific concentration
CAS number
9002-04-4
RTECS
XO8950000
Specific activity
≥1800 NIH units/mg protein
Activity
≥50 NIH units/mg dry weight
Unit definition
One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.
Solubility
50 mM NaCl (≤2000 NIH units/ml) or H₂O (>2000 NIH units/ml)
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 2 months at -70°C.
From Catalog: Desc. Field- added "Key enzyme that activates factor XIII and converts fibronogen into fibrin.
