Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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539202
Sigma-AldrichProtein A, Staphylococcus aureus
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Description
Overview
Native protein A from Staphylococcus aureus. Provides a versatile and economical means for isolating, detecting, and purifying antibodies. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors and, therefore, will not give high background reactions. Strongly reactive with guinea pig; human IgG1, IgG2, and IgG4; mouse IgG2b, and rabbit. Weakly reactive with bovine; human IgA, IgE, and IgM; and mouse IgG3. Nonreactive with goat; horse; human IgD and IgG3; rat; mouse IgG1; and sheep.
Protein A, Staphylococcus aureus Certificates of Analysis
Title
Lot Number
539202
References
Reference overview
Sjødahl, J. 1976. FEBS Lett.67, 1976.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
04-September-2008 RFH
Description
Protein A, a component of the cell wall of Staphylococcus aureus, is sometimes called the universal second step reagent because it binds to the Fc portion of most immunoglobulin classes. It provides a versatile and economical means for isolating, detecting, and purifying antibodies. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors and, therefore, will not give high background reactions. Strongly reactive with guinea pig; human IgG1, IgG2 and IgG4; and rabbit. Weakly reactive with bovine; human IgA, IgE and IgM; and mouse.Nonreactive with goat; horse; human IgD and IgG3; rat; and sheep.
Form
Lyophilized solid
Solubility
H₂O
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
