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14-845 PIP5K1γ Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant human PIP5K1γ, full length. For use in Kinase Assays.

PIP5K1γ Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-845
10 µg  Also available in 250μg size as catalog # 14-845M - Call for pricing and availability.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-845
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPIP5K1γ Protein, active, 10 µg
      OverviewN-terminal 6His-tagged, recombinant human PIP5K1γ, full length.
      References
      Product Information
      Presentation10µg of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal 6His-tagged, recombinant human PIP5K1γ, full length. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.
      Gene Symbol
      • PIP5K1C
      • KIAA0589
      Protein TargetPIP5K1γ
      Purification MethodImmobilized metal affinity chromatography (IMAC)
      Target Sub-FamilyPIPK
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse. Mediates RAC1-dependent reorganization of actin filaments. Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
      Enzyme regulation: Activated by interaction with TLN2.
      SUBUNIT STRUCTURE: Interacts with TLN1 and CSK. Interacts with TLN2 and ARF6. Interacts with AP2B1. Interacts with AP2M1; phosphorylation of PIP5K1C by CSK disrupts the interaction; clathrin competes with PIP5K1C.
      SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations.
      INVOLVEMENT IN DISEASE: Defects in PIP5K1C are the cause of lethal congenital contracture syndrome type 3 (LCCS3) [MIM:611369]; also known as multiple contractural syndrome Israeli Bedouin type B. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, the Pena-Shokeir phenotype, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS3 patients present at birth with severe multiple joint contractures with severe muscle wasting and atrophy, mainly in the legs. LCCS3 can be distinguished from the original LCCS by the absence of hydrops, fractures, and multiple pterygia.
      SEQUENCE SIMILARITIES: Contains 1 PIPK domain.
      Molecular Weight77kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in the PIProfiler.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size as catalog # 14-845M - Call for pricing and availability.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-845 04053252473883

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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases