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33-125 PIP5-Kinase HTRF Assay

The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.

PIP5-Kinase HTRF Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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33-125
384 wells  Kit capacity: 384 wells
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      Overview

      Replacement Information
      Description
      Catalogue Number33-125
      Brand Family Upstate
      Trade Name
      • HTRF
      • Upstate
      DescriptionPIP5-Kinase HTRF Assay
      References
      Product Information
      Components
      • Reaction Buffer (4X)
      • DM A Detection Reagent
      • DM C Detection Reagent
      • DM E Detection Reagent
      • Phosphatidylinositol 4-phosphate (1mM)
      • Phosphatidylserine (1mM)
      • Stop Reagent D
      Detection methodHTRF
      Quality LevelMQ100
      Applications
      ApplicationThe PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 384 well format. Suitable for screening of potential inhibitors of these enzymes.
      Key Applications
      • Kinase Assay
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      Analytes Available
      • PIP5 Kinase
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 4-phosphate 5-kinase type-1 alpha
      catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs.
      Gene Symbol
      • PIP5K1α
      • PIP5K1γ
      Target Sub-FamilyPI3K/PI4K
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions12 months at -70°C
      Packaging Information
      Material Size384 wells
      Material PackageKit capacity: 384 wells
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      33-125 04053252374722

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