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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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480012
MilliporeNDSB Set - Calbiochem
Contains the following non-detergent sulfobetaines: 5 g of NDSB-195, 25 g of NDSB-201, and 5 g of NDSB-256.
More>>Contains the following non-detergent sulfobetaines: 5 g of NDSB-195, 25 g of NDSB-201, and 5 g of NDSB-256. Less<<
NDSB Set - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Contains the following non-detergent sulfobetaines: 5 g of NDSB-195 (Cat. No. 480001), 25 g of NDSB-201 (Cat. No. 480005), and 5 g of NDSB-256 (Cat. No. 480010). Supplied with a data sheet.
Catalogue Number
480012
Brand Family
Calbiochem®
References
References
Goldberg, M.E., et al. 1996. Folding and Design1, 21. Goldberg, M.E., et al. 1995. Folding & Design1, 21. Vuillard, L., et al. 1995. Biochem. J.305, 337. Vuillard, L., et al. 1995. Anal. Biochem.230, 290. Vuillard, L., et al. 1994. FEBS Lett.353, 294.
Goldberg, M.E., et al. 1996. Folding and Design1, 21. Goldberg, M.E., et al. 1995. Folding & Design1, 21. Vuillard, L., et al. 1995. Biochem. J.305, 337. Vuillard, L., et al. 1995. Anal. Biochem.230, 290. Vuillard, L., et al. 1994. FEBS Lett.353, 294.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
11-August-2008 RFH
Description
Non-detergent sulfobetaines (NDSBs) are zwitterionic compounds. Like zwittergents (zwitterionic detergents), NDSBs carry the sulfobetaine hydrophilic head group. However, unlike zwittergents, the hydrophobic group in NDSBs is too short for micellar formation even at concentrations as high as 1M. Hence, NDSBs do not behave like detergents. Because of their zwitterionic nature, NDSBs were first employed to neutralize electrostatic interactions without increasing the conductivity in native isoelectrofocusing gels. Recently they have found utility in various other applications including isolation of membrane proteins, purification of nuclear proteins and halophilic proteins, renaturation of chemically and thermally denatured proteins and protein crystallization. They are potentially useful in solubilization and renaturation of fusion proteins from inclusion bodies. It is hypothesized that the hydrophobic group, although short, interacts with the hydrophobic regions of the protein preventing aggregation during renaturation. Also, presumably, the contribution from the short hydrophobic groups combined with charge neutralization ability of the sulfobetaine group results in higher yields of membrane proteins.
NDSBs do not interfere with enzymatic assays based on chromogenic substrates bearing nitrophenyl groups and do not inhibit the activities of β-galactosidase and alkaline phosphatase, the two enzymes tested thus far. NDSB-195 has the added advantage in that it does not absorb at 280 nm, making it compatible with protein purification procedures in which the protein concentrations are determined by absorbance measurements at 280 nm. Supplied with a data sheet.
Form
Solid
Solubility
H₂O (50 mg/ml)
Storage
+15°C to +30°C Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Store lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).
Toxicity
Irritant
References
Goldberg, M.E., et al. 1996. Folding and Design1, 21. Goldberg, M.E., et al. 1995. Folding & Design1, 21. Vuillard, L., et al. 1995. Biochem. J.305, 337. Vuillard, L., et al. 1995. Anal. Biochem.230, 290. Vuillard, L., et al. 1994. FEBS Lett.353, 294.