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FC010-5MG Human Plasma Fibronectin Purified Protein

Human Plasma Fibronectin Purified Protein MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture
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FC010-5MG
5 mg  5 x 1 mg
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      Overview

      Key Spec Table

      Key Applications
      CULT
      Description
      Catalogue NumberFC010-5MG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Plasma Fibronectin Purified Protein
      OverviewHuman fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
      Product Information
      PresentationLiquid in 50 mM tris-buffered saline (TBS), pH 7.5, containing no preservatives.
      Quality LevelMQ100
      Applications
      Key Applications
      • Cell Culture
      Application NotesImmunochemical standard

      Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

      Suggested Procedure for Coating Cell Cultureware

      1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.

      2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

      3. Introduce the proper CO2 atmosphere, if required.

      4. Add the calculated amount of HFN to each culture vessel.

      5. Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

      6. Remove residual balanced salt solution before proceeding with standard cell culture procedures
      Biological Information
      Concentration1 mg/mL
      PurityApproximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
      SourceHuman donor plasma.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
      Gene Symbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
      SIZE: 2386 amino acids; 262607 Da
      SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
      PTM: Sulfated.
      SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.
      Packaging Information
      Material Size5 mg
      Material Package5 x 1 mg
      Global Trade Item Number
      Catalogue Number GTIN
      FC010-5MG 04053252331183