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109204 Giemsa's azur eosin methylene blue solution

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109204
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1092040100
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      Glass bottle 100 ml
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      1092040500
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          Plastic bottle 500 ml
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          1092041000
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              Plastic bottle 1 l
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              1092042500
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                  Plastic bottle 2.5 l
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                  1092049025
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                      Barrel PE/met 25 l
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                      Description
                      Catalogue Number109204
                      DescriptionGiemsa's azur-eosin-methylene blue solution
                      OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                      References
                      Product Information
                      HS Code3212 90 90
                      Quality LevelMQ400
                      Applications
                      Applicationfor microscopy
                      Biological Information
                      Physicochemical Information
                      Boiling point>65 °C (1013 hPa)
                      Density0.99 g/cm3 (20 °C)
                      Explosion limit5.5 - 44 %(V) Methanol
                      Flash point18 °C
                      Ignition temperature455 °C
                      pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
                      Dimensions
                      Materials Information
                      Toxicological Information
                      Safety Information according to GHS
                      Hazard Pictogram(s) Hazard Pictogram(s)
                      Hazard Pictogram(s)
                      Hazard Pictogram(s)
                      Hazard Statement(s)H225: Highly flammable liquid and vapour.
                      H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
                      H317: May cause an allergic skin reaction.
                      H370: Causes damage to organs.
                      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
                      P233: Keep container tightly closed.
                      P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
                      P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
                      P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
                      P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
                      P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
                      Signal WordDanger
                      Storage class3 Flammable liquids
                      WGKWGK 2 obviously hazardous to water
                      Disposal9
                      Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
                      Safety Information
                      Categories of dangerhighly flammable, toxic
                      Product Usage Statements
                      Storage and Shipping Information
                      StorageStore at +15°C to +25°C.
                      Packaging Information
                      Transport Information
                      Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
                      Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
                      Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
                      Supplemental Information
                      Specifications
                      Suitability for microscopypasses test
                      Erythrocytesreddish-brownish
                      Nuclei / Chromatinred to violet
                      Eosinophilic granulesreddish to red-brown
                      Neutrophilic granuleslight violet
                      Lymphocyte cytoplasmnblue
                      Monocyte cytoplasmgrey-blue
                      Thrombocytesviolet
                      Basophilic granulesdark violet
                      Nuclei (Tissue section)blue to dark blue
                      Cellsblue to dark blue
                      Collagenpale blue
                      Osteoidpale blue
                      Eosinophilic granula (Tissue section)red
                      Acidic mucopolysaccharidesreddish-violet
                      Mast cell granulesreddish-violet
                      Cartilage matrixreddish-violet
                      Acidophilic materialsorange-red
                      Helicobacter pylori
                      blue to dark blue
                      blue to dark blue
                      Global Trade Item Number
                      Catalogue Number GTIN
                      1092040100 04022536111841
                      1092040500 04022536111889
                      1092041000 04022536720081
                      1092042500 04022536111926
                      1092049025 04022536111940

                      Documentation

                      Giemsa's azur eosin methylene blue solution SDS

                      Title

                      Safety Data Sheet (SDS) 

                      Giemsa's azur eosin methylene blue solution Certificates of Analysis

                      Product Number PackagingSpecificationLot Number
                      1092040100 Glass bottlePDF Specification Document
                      1092040500 Plastic bottlePDF Specification Document
                      1092041000 Plastic bottlePDF Specification Document
                      1092042500 Plastic bottlePDF Specification Document
                      1092049025 Barrel PE/metPDF Specification Document

                      User Guides

                      Title
                      User Guide (IFU) - 109204

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                      Categories

                      IVD/OEM Materials & Reagents > Microscopy > Hematology > Rapid Stainings, Hemacolor®
                      IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Solutions
                      IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents
                      IVD/OEM Materials & Reagents > Microscopy > Hematology > Hematological Staining Solutions
                      IVD/OEM Materials & Reagents > Microscopy > Bacteriology > General Bacteriological Staining Solutions