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69036 Factor Xa, Restriction Grade, Bovine Plasma

Factor Xa, Restriction Grade, Bovine Plasma MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69036
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRestriction Grade Factor Xa is a highly purified preparation isolated from bovine plasma and activated with Russell's viper venom. This preparation is purified to near homogeneity and shows no secondary cleavage from contaminating proteases. The preparation is also functionally tested for activity with fusion proteins. Like enterokinase, Factor Xa cleaves at the C-terminal side of the recognition sequence (IleGluGlyArg↓) and can therefore be used for removing all vector-encoded sequences from appropriately designed constructs.

      Specific Activity: ≥150 IU/mg

      One IU is defined as the amount of enzyme that will release 1 µmole of p-nitroaniline per minute using methanesulfonyl-D-leucyl-glycyl-arginyl-paranitroanilide at 37°C, pH 8.4.

      One µg of enzyme cleaves 50 µg Cleavage Control Protein to >95% completion in 16 hr at 25°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl2.
      Catalogue Number69036
      Brand Family Novagen®
      Product Information
      •100 µgRestriction Grade Factor Xa
      •2 mlFactor Xa Dilution/Storage Buffer
      •1 ml10X Factor Xa Cleavage Buffer
      •10 µgCleavage Control Protein
      Quality LevelMQ100
      Biological Information
      Biological activityOne microgram of enzyme cleaves 50 µg Xa Cleavage Control Protein to >95% completion in 16 hours at 25°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl₂.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      69036-3 04055977259636