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204888 Complement C5, Human

Complement C5, Human, CAS 80295-53-0. is a native glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds.

Complement C5, Human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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204888
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Overview

Replacement Information

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204888-250UG
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      		 Plastic ampoule 250 μg
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      Description
      OverviewNative, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in the assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at peptide bond 74 resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form lytic C5b-9 complement membrane attack complex (MAC).
      Catalogue Number204888
      Brand Family Calbiochem®
      References
      ReferencesJanatova, J. 1988. Methods Enzymol. 162, 579.
      Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.
      Product Information
      CAS number80295-53-0
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Quality LevelMQ200
      Applications
      Biological Information
      Biological activity≥100,000 C₅H₅0 units/mg; ≥60% of the C5 activity in normal human serum on a mg/mg basis
      Purity≥95% by SDS-PAGE
      SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsIgG, IgA, IgM, albumin, ceruloplasmin or functionally C3, C6, C7, C8, or C9: ≤trace amounts
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      204888-250UG 04055977219814

      Documentation

      Complement C5, Human SDS

      Title

      Safety Data Sheet (SDS) 

      Complement C5, Human Certificates of Analysis

      TitleLot Number
      204888

      References

      Reference overview
      Janatova, J. 1988. Methods Enzymol. 162, 579.
      Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.

      Citations

      Title
    • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-May-2008 RFH
      DescriptionNative, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120,000 (α) and 75,000 (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at the peptide bond 74, resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form the lytic C5b-9 complement membrane attack complex (MAC).
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      CAS number80295-53-0
      Purity≥95% by SDS-PAGE
      ContaminantsIgG, IgA, IgM, albumin, ceruloplasmin or functionally C3, C6, C7, C8, or C9: ≤trace amounts
      Biological activity≥100,000 C₅H₅0 units/mg; ≥60% of the C5 activity in normal human serum on a mg/mg basis
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesJanatova, J. 1988. Methods Enzymol. 162, 579.
      Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.
      Citation
    • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.
      		•