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233155 MilliporeCleland’s Reagent - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Brochures
Data Sheet

Synonyms: DTT, Dithiothreitol

Recommended Products

Overview

Replacement Information

Key Spec Table

CAS #	Empirical Formula
3483-12-3	C₄H₁₀O₂S₂

Pricing & Availability

Catalogue Number	Availability	Packaging	Qty/Pack	Price	Quantity
233155-100GM	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Glass bottle	100 gm	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
233155-10GM	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic ampoule	10 gm	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
233155-25GM	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Glass bottle	25 gm	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
233155-5GM	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic ampoule	5 gm	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites

Description
Overview	A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.
Overview	Oxidized dithiothreitol: ≤0.5%.
Catalogue Number	233155
Brand Family	Calbiochem®
Synonyms	DTT, Dithiothreitol

References
References	Gailis, L. 1994. Eur. J. Pharmacol. 292, 39. Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125. Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199. Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716. Cleland, W.W. 1964. Biochemistry 3, 480.

Product Information
CAS number	3483-12-3
Form	White to off-white powder
Hill Formula	C₄H₁₀O₂S₂
Chemical formula	C₄H₁₀O₂S₂
Quality Level	MQ100

Applications

Biological Information
Purity	≥97% by titration

Physicochemical Information
Contaminants	Heavy metals: ≤1 ppm
Heavy metals	<1 ppm

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	EK1610000

Safety Information
R Phrase	R: 22-36/38 Harmful if swallowed. Irritating to eyes and skin.
S Phrase	S: 36-26 Wear suitable protective clothing. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Harmful
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
233155-100GM	07790788054274
233155-10GM	04055977217698
233155-25GM	04055977217711
233155-5GM	04055977217728

Documentation

Cleland’s Reagent - CAS 3483-12-3 - Calbiochem SDS

Title
Safety Data Sheet (SDS)

Cleland’s Reagent - CAS 3483-12-3 - Calbiochem Certificates of Analysis

Title	Lot Number
233155	Enter Lot Number

References

Reference overview
Gailis, L. 1994. Eur. J. Pharmacol. 292, 39. Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125. Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199. Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716. Cleland, W.W. 1964. Biochemistry 3, 480.

Brochure

Title
Bulk Product Guide

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	29-April-2008 RFH
Synonyms	DTT, Dithiothreitol
Description	DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL^™ Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.
Form	White to off-white powder
Intert gas (Yes/No)	Packaged under inert gas
CAS number	3483-12-3
RTECS	EK1610000
Chemical formula	C₄H₁₀O₂S₂
Purity	≥97% by titration
Contaminants	Heavy metals: ≤1 ppm
Solubility	Ethanol or H₂O (30 mg/ml)
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Harmful
Merck USA index	14, 3376
References	Gailis, L. 1994. Eur. J. Pharmacol. 292, 39. Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125. Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199. Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716. Cleland, W.W. 1964. Biochemistry 3, 480.

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