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208771 Calpain Substrate III, Fluorogenic - Calbiochem

A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (DABCYL)-TPLK~SPPPSPR-(EDANS)

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208771
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Overview

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208771-1MG
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      Description
      OverviewA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
      Catalogue Number208771
      Brand Family Calbiochem®
      Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
      References
      ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
      Product Information
      ATP CompetitiveN
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₈₀H₁₁₄N₂₀O₁₈S
      Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
      ReversibleN
      Quality LevelMQ100
      Biological Information
      Primary TargetSubstrate sequence that is optimized for calpain-1 and -2
      Primary Target IC<sub>50</sub>kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Global Trade Item Number
      Catalogue Number GTIN
      208771-1MG 04055977202854