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OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).

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OP33
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, Pm, RMMonoclonal Antibody

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OP33-20UG
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      Description
      OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
      Catalogue NumberOP33
      Brand Family Calbiochem®
      References
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Ball, R.K., et al. 1984. EMBO J. 3, 1485.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlHs27 cells or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
      Key Applications Frozen Sections
      Immunofluorescence
      Immunoprecipitation
      Not Western Blot
      Paraffin Sections
      Application NotesFrozen Sections (see applications references)
      Immunoblotting (not recommended)
      Immunofluorescence (1-20 μg/ml)
      Immunoprecipitation (1 μg per sample)
      Paraffin Sections (5 μg/ml, heat pre-treatment required)
      Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenvLM mouse tumor cells
      ImmunogenMouse
      ClonePAb1620
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Primate
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      OP33-20UG 07790788053901