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OP23 Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1)

This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP23
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
OP23-200UG
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      		 Plastic ampoule 200 μg
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      Description
      OverviewRecognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.
      Catalogue NumberOP23
      Brand Family Calbiochem®
      References
      ReferencesRodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
      Positive controlNormal skin tissue or ras 1 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (5 µg/ml)
      Immunoblotting (10 µg/ml)
      Immunofluorescence (2.5 µg/ml)
      Immunoprecipitation (5 µg/ml)
      Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
      Application CommentsFor immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant p21 Ras
      Epitopewithin amino acids 54-188
      CloneF235-1.7.1
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP23-200UG 04055977208986

      Documentation

      Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) Certificates of Analysis

      TitleLot Number
      OP23

      References

      Reference overview
      Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationFrozen Sections (5 µg/ml)
      Immunoblotting (10 µg/ml)
      Immunofluorescence (2.5 µg/ml)
      Immunoprecipitation (5 µg/ml)
      Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.
      BackgroundThe human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-Ras are the homologues of v-H- and v-K-Ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and their relevance to human disease diagnosis and prognosis is still emerging.
      HostMouse
      Immunogenrecombinant p21 Ras
      Epitopewithin amino acids 54-188
      CloneF235-1.7.1
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlNormal skin tissue or ras 1 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesRodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.