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OP14 Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu

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OP14
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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OP14-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
      Catalogue NumberOP14
      Brand Family Calbiochem®
      SynonymsAnti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu
      References
      ReferencesVan De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al.1987 Science 237, 178.
      Slamon, D.J., et al. 1987 Science 235, 177.
      Varley, J.M., et al.1987 Oncogene 1, 423.
      Bargmann, C.I., et al. 1986 Nature 319, 226.
      Yamamoto, T., et al.1986 Nature 319, 230.
      Blick, M., et al. 1984 Blood 64, 1234.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlNormal skin tissue or HepG2 cells
      Positive controlSK-BR-3 cells or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
      Key Applications Frozen Sections
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (see application references)
      Application CommentsImmunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogencells overexpressing human c-ErbB2/c-Neu
      ImmunogenHuman
      Clone9G6
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP14-100UG 04055977224863

      Documentation

      Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) Certificates of Analysis

      TitleLot Number
      OP14

      References

      Reference overview
      Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al.1987 Science 237, 178.
      Slamon, D.J., et al. 1987 Science 235, 177.
      Varley, J.M., et al.1987 Oncogene 1, 423.
      Bargmann, C.I., et al. 1986 Nature 319, 226.
      Yamamoto, T., et al.1986 Nature 319, 230.
      Blick, M., et al. 1984 Blood 64, 1234.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-June-2007 RFH
      SynonymsAnti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu
      ApplicationFrozen Sections (5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (see application references)
      DescriptionThis clone expresses the kappa light chain isotype. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.
      BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
      HostMouse
      Immunogen speciesHuman
      Immunogencells overexpressing human c-ErbB2/c-Neu
      Clone9G6
      IsotypeIgG₁
      Specieshuman
      Positive controlSK-BR-3 cells or breast carcinoma tissue
      Negative controlNormal skin tissue or HepG2 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsImmunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesVan De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al.1987 Science 237, 178.
      Slamon, D.J., et al. 1987 Science 235, 177.
      Varley, J.M., et al.1987 Oncogene 1, 423.
      Bargmann, C.I., et al. 1986 Nature 319, 226.
      Yamamoto, T., et al.1986 Nature 319, 230.
      Blick, M., et al. 1984 Blood 64, 1234.
      Application referencesParaffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.