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574777 Anti-Synapsin I Rabbit pAb

This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.

Anti-Synapsin I Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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574777
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RRbPolyclonal Antibody

Pricing & Availability

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574777-10UG
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      		 Plastic ampoule 10 μg
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      Description
      OverviewRecognizes the synapsin I protein in rat brain.
      Catalogue Number574777
      Brand Family Calbiochem®
      References
      ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
      Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
      Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
      Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
      Product Information
      FormLyophilized
      FormulationLyophilized from 5 mM ammonium bicarbonate.
      Positive controlRat brain
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesFrozen Sections Andersson, S., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 3857.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesELISA (1:2500-1:10,000)
      Frozen Sections (see application references)
      Immunoblotting (1:200)
      Immunocytochemistry (1:500-1:2500)
      Immunoprecipitation (1 µg/200 µg lysate)
      Application CommentsThis antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified, brovine brain synapsin I
      ImmunogenBovine
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      574777-10UG 04055977189735

      Documentation

      Anti-Synapsin I Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Synapsin I Rabbit pAb Certificates of Analysis

      TitleLot Number
      574777

      References

      Reference overview
      Stone, L.M., et al. 1994. J. Neurosci. 14, 301.
      Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
      Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
      Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2007 RFH
      ApplicationELISA (1:2500-1:10,000)
      Frozen Sections (see application references)
      Immunoblotting (1:200)
      Immunocytochemistry (1:500-1:2500)
      Immunoprecipitation (1 µg/200 µg lysate)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.
      BackgroundSynapsin I is a neuron-specific protein that serves as an excellent marker for synaptic terminals.
      HostRabbit
      Immunogen speciesBovine
      Immunogenpurified, brovine brain synapsin I
      IsotypeIgG
      Speciesbovine, human, mouse, rat
      Positive controlRat brain
      FormLyophilized
      FormulationLyophilized from 5 mM ammonium bicarbonate.
      PreservativeNone
      CommentsThis antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
      Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
      Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
      Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
      Application referencesFrozen Sections Andersson, S., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 3857.