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AB5539 Anti-Neurofilament H Antibody

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AB5539
50 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, R, FeICC, IHC, IH(P), WBChPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5539
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament H Antibody
      Alternate Names
      • NF-H
      Product Information
      FormatPurified
      Control
      • Positive control: brain.

        Negative control: kidney, liver.
      PresentationPurified chicken IgY in PBS containing 5mM sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.

      Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.

      Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified bovine NF-H.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostChicken
      SpecificityRecognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      • Feline
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFH
      • NF-H
      • NFH
      • KIAA0845
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
      SIZE: 1026 amino acids; 112480 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
      SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
      Molecular Weight200 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µL
      Global Trade Item Number
      Catalogue Number GTIN
      AB5539 04053252506338