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MAB1951F Anti-Integrin β1 Antibody, clone P4G11, FITC conjugated

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MAB1951F
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, RbFC, IF, IHCMFITCMonoclonal Antibody
      Description
      Catalogue NumberMAB1951F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone P4G11, FITC conjugated
      Alternate Names
      • CD29
      References
      Product Information
      FormatFITC
      HS Code3002 15 90
      PresentationFluorescein-conjugated protein A purified immunoglobulin in 0.02M PBS, 0.25M NaCl, pH 7.6, with 0.1% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationDetect Integrin β1 using this Anti-Integrin β1 Antibody, clone P4G11, FITC conjugated validated for use in FC, IF, IH.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesMAB1951F has been designed primarily for FACS analysis on live or lightly paraformaldehyde cells. Ca++ must be present in all solutions to maintain antibody contact with the antigen. Typical dilution is 1:100 for 1 x10(6) cells. Washes using complete PBS (containing Ca++ and Mg++) and 1% BSA are recommended.



      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman IL-1beta activated endothelial cells
      CloneP4G11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityHuman integrin beta1. The epitope recognized by MAB1951F is Ca2+dependent.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
      Gene Symbol
      • ITGB1
      • GPIIA
      • OTTHUMP00000063731
      • OTTHUMP00000046253
      • OTTHUMP00000063733
      • VLAB
      • OTTHUMP00000063732
      • MDF2
      • FNRB
      • CD29
      • MSK12
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


      SIZE: 798 amino acids; 88465 Da

      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

      TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).





      SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws. Keep away from light
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1951F 04053252503047

      Documentation

      Anti-Integrin β1 Antibody, clone P4G11, FITC conjugated SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin β1 Antibody, clone P4G11, FITC conjugated Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) FLUORESCEIN-CONJUGATED MONOCLONAL ANTIBODY - 2451379 2451379
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) - 3192049 3192049
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) - 3993558 3993558
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) - 4042591 4042591
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) -2615032 2615032
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) -2746275 2746275
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) FLUORESCEIN-CONJUGATED MONOCLONAL ANTIBODY 2949181
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) FLUORESCEIN-CONJUGATED MONOCLONAL ANTIBODY 3074354
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) FLUORESCEIN-CONJUGATED MONOCLONAL ANTIBODY 2935883
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) FLUORESCEIN-CONJUGATED MONOCLONAL ANTIBODY - 1996992 1996992

      References

      Reference overviewPub Med ID
      OmpA-mediated rickettsial adherence to and invasion of human endothelial cells is dependent upon interaction with α2β1 integrin.
      Hillman, RD; Baktash, YM; Martinez, JJ
      Cellular microbiology  15  727-41  2013

      Show Abstract
      23145974 23145974
      Hormonal regulation of galectin 3 in trophoblasts and its effects on endometrium.
      Huan Yang,Hugh S Taylor,Caixia Lei,Chao Cheng,Wei Zhang
      Reproductive sciences (Thousand Oaks, Calif.)  18  2011

      Show Abstract
      21555510 21555510
      Assessment of the integrin alpha5beta1 antagonist JSM6427 in proliferative vitreoretinopathy using in vitro assays and a rabbit model of retinal detachment.
      Zahn, G; Volk, K; Lewis, GP; Vossmeyer, D; Stragies, R; Heier, JS; Daniel, PE; Adamis, AP; Chapin, EA; Fisher, SK; Holz, FG; Löffler, KU; Knolle, J
      Investigative ophthalmology & visual science  51  1028-35  2010

      Show Abstract
      19815730 19815730
      Interactions between galectin-3 and integrinbeta3 in regulating endometrial cell proliferation and adhesion.
      Lei CX, Zhang W, Zhou JP, Liu YK
      Human reproduction (Oxford, England)  24  2879-89  2009

      Show Abstract
      19633306 19633306
      Accumulation of acetylcholine receptors is a necessary condition for normal accumulation of acetylcholinesterase during in vitro neuromuscular synaptogenesis.
      De La Porte, S, et al.
      Eur. J. Neurosci., 10: 1631-43 (1998)  1998

      Show Abstract
      9751136 9751136
      Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
      Wayner, E A, et al.
      J. Cell Biol., 121: 1141-52 (1993)  1993

      Show Abstract
      8501119 8501119

      Data Sheet

      Title
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) FLUORESCEIN-CONJUGATED MONOCLONAL ANTIBODY

      Related Products & Applications

      Alternative Format

      Catalogue Number Description  
      MAB1951Z Anti-Integrin β1 Antibody, clone P4G11, azide free Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies