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371729 Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb

Anti-Giα-3-Subunit, C-Terminal (345-354), rabbit polyclonal, recognizes Giα-3 subunit. Does not cross-react with Gsα & Goα subunits. Validated for use in WB & IP.

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371729
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
MaRbPolyclonal Antibody

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371729-50UL
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      Description
      OverviewRecognizes Giα-3 subunit. Does not cross-react with Gsα and Goα, but partially cross-reacts with Giα1-, G1α2-subunits.
      Catalogue Number371729
      Brand Family Calbiochem®
      References
      ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
      Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
      Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem. 262, 14,241.
      Product Information
      FormLiquid
      FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (see comments)
      Application CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mammals
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      371729-50UL 04055977213300