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GR15L Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epidermal Growth Factor Receptor

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GR15L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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GR15L-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~170 kDa EGF receptor protein in A431 cells.
      Catalogue NumberGR15L
      Brand Family Calbiochem®
      SynonymsAnti-Epidermal Growth Factor Receptor
      Application Data
      Detection of human EGFR by immunocytochemistry. Sample: A431 cells fixed with methanol. Primary Antibody: Anti-Epidermal Growth Factor Receptor (Ab-5) Mouse mAb (Cat. No. GR15L) (1 µg/ml). Detection: fluorescence.
      References
      ReferencesHunter T., 1984. Nature 311, 414.
      Reynolds, F.H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlHL-60 cells
      Positive controlA-431 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesNeutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511.
      Key Applications Flow Cytometry
      Immunofluorescence
      Immunoprecipitation
      Neutralization Studies
      Application NotesFlow Cytometry (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Neutralization Studies (1-2 µg/ml; see application references)
      Application CommentsStains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human EGFR from A431 cells
      ImmunogenHuman
      CloneEGFR.1
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR15L-100UG 04055977228250

      Documentation

      Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) Certificates of Analysis

      TitleLot Number
      GR15L

      References

      Reference overview
      Hunter T., 1984. Nature 311, 414.
      Reynolds, F.H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-Epidermal Growth Factor Receptor
      ApplicationFlow Cytometry (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Neutralization Studies (1-2 µg/ml; see application references)
      Application Data
      Detection of human EGFR by immunocytochemistry. Sample: A431 cells fixed with methanol. Primary Antibody: Anti-Epidermal Growth Factor Receptor (Ab-5) Mouse mAb (Cat. No. GR15L) (1 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
      BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor alpha to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
      HostMouse
      Immunogen speciesHuman
      Immunogenpartially purified, human EGFR from A431 cells
      CloneEGFR.1
      IsotypeIgG2b
      Specieshuman, not mouse, not rat
      Positive controlA-431 cells
      Negative controlHL-60 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsStains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
      Toxicity Standard Handling
      ReferencesHunter T., 1984. Nature 311, 414.
      Reynolds, F.H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Application referencesNeutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511.