Millipore Sigma Vibrant Logo

616376 TRAIL, Human, Recombinant, NSO cells

TRAIL, Human, Recombinant, NSO cells : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Apo2L; TNF-Related Apoptosis-Inducing Ligand

View Products on Sigmaaldrich.com
616376
Voir les Prix & la Disponibilité

Aperçu

Replacement Information

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
616376-10UG
Récupération des données relatives à la disponibilité...
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      		 Ampoule plast. 10 μg
      Prix en cours de récupération
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      —
      Demander le prix
      		 —
      Description
      OverviewRecombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.
      Catalogue Number616376
      Brand Family Calbiochem®
      SynonymsApo2L; TNF-Related Apoptosis-Inducing Ligand
      References
      ReferencesGriffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
      Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
      Pan, G., et al. 1997. Science 277, 815.
      Pan, G., et al. 1997. Science 276, 111.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/µg TRAIL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      616376-10UG 04055977186253

      Documentation

      TRAIL, Human, Recombinant, NSO cells FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      TRAIL, Human, Recombinant, NSO cells Certificats d'analyse

      TitreNuméro de lot
      616376

      Références bibliographiques

      Aperçu de la référence bibliographique
      Griffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
      Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
      Pan, G., et al. 1997. Science 277, 815.
      Pan, G., et al. 1997. Science 276, 111.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.

      Brochure

      Titre
      Caspases and other Apoptosis Related Tools Brochure

      Citations

      Titre
    • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
    • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.
      		•
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-September-2008 RFH
      SynonymsApo2L; TNF-Related Apoptosis-Inducing Ligand
      DescriptionRecombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/µg TRAIL
      Biological activityED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
      SolubilityReconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesGriffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
      Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
      Pan, G., et al. 1997. Science 277, 815.
      Pan, G., et al. 1997. Science 276, 111.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
      Citation
    • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
    • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.
      		•