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481974 NADPH-P450 Oxidoreductase, Human, Recombinant, S. frugiperda

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481974
  
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      Aperçu

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratio 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.

      Catalogue Number481974
      Brand Family Calbiochem®
      References
      ReferencesShen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
      Yamano, S., et al. 1989. Mol. Pharmacol. 36, 83.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will oxidize 1 µmol of cytochrome <i>c</i> per min at 20°C, pH 7.7.
      EC number1.6.2.4
      FormLiquid
      FormulationIn 10 mM potassium phosphate buffer, 1 mM DTT, 100 µM EDTA, 20% glycerol, pH 7.7.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥20 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      481974 0

      Documentation

      NADPH-P450 Oxidoreductase, Human, Recombinant, S. frugiperda Certificats d'analyse

      TitreNuméro de lot
      481974

      Références bibliographiques

      Aperçu de la référence bibliographique
      Shen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
      Yamano, S., et al. 1989. Mol. Pharmacol. 36, 83.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-May-2008 RFH
      DescriptionRecombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane-bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratios ranging from 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.
      FormLiquid
      FormulationIn 10 mM potassium phosphate buffer, 1 mM DTT, 100 µM EDTA, 20% glycerol, pH 7.7.
      Concentration Label Please refer to vial label for lot-specific concentration
      EC number1.6.2.4
      Purity≥90% by SDS-PAGE
      Specific activity≥20 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will oxidize 1 µmol of cytochrome c per min at 20°C, pH 7.7.
      SolubilityAqueous buffers
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesShen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
      Yamano, S., et al. 1989. Mol. Pharmacol. 36, 83.