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HCY4MG-64K-PX20 MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel IV, Premixed 20 Plex Assay

The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Premix Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 20 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.

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HCY4MG-64K-PX20
96-well plate  96 well plate
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      Replacement Information
      Description
      Catalogue NumberHCY4MG-64K-PX20
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel IV, Premixed 20 Plex Assay
      OverviewEMD Millipore’s MILLIPLEX® MAP Cytokine/Chemokine Magnetic Bead Panel IV Premix is a 20-plex premixed kit to be used for the simultaneous quantification of all of the following analytes in serum, plasma or culture supernatant samples: BAFF/Blys, BRAK/CSCL14, CCL28, CXCL16, HMGB1, IFNβ, IL-14, IL-19, IL-24, IL-28B, IL-32α, IL-34, IL-35, IL-36β, IL-37, IL-38, MIP-4/PARC/CCL18, MPIF-1/CL23 and YKL40/CHI3L1.

      MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

      Each MILLIPLEX® MAP panel and kit includes:
      • Quality controls (QCs) provided to qualify assay performance

      • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

      • Optimized serum matrix to mimic native analyte environment

      • Detection antibody cocktails designed to yield consistent analyte profiles within panel


      EMD Millipore’s MILLIPLEX® MAP Human Cytokine/Chemokine Magnetic Bead Panel IV is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
      Background InformationInflammation and immune cell response are key to maintaining homeostasis. The more we understand mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease: certainly immunological diseases, such as allergic reactions, asthma, infectious disease and an ever-growing number of conditions under the autoimmune umbrella, but also neurodegenerative disease, metabolic and cardiovascular disease and even cancer. The ability to measure multiple inflammation biomarkers simultaneously using EMD Millipore’s MILLIPLEX ® MAP panels, based on the Luminex xMAP® technology, to establish disease profiles that can be used for screening, diagnosis and response to treatment is critical for understanding the pathogenesis of these diseases.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-assay: 10
      • Inter-assay:15-25
      Panel TypeMAGNETIC Cytokines/Chemokines
      Applications
      ApplicationThe Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Premix Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 20 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.

      • A maximum of 25 μL per well of 1:4 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay. Please note that HMGB1 can be measured in serum and plasma samples only.
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • BAFF/Blys
      • BRAK/CXCL14
      • CCL28
      • CXCL16
      • HCC-4/CCL16
      • HMGB1
      • IFNβ
      • IL-14
      • IL-19
      • IL-24
      • IL-28B
      • IL-36β/IL-1F8
      • IL-32α
      • IL-34
      • IL-35
      • IL-37/IL-1F7
      • IL-38/IL-1F10
      • MIP-4
      • MPIF-1/CCL23
      • YKL40
      Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Accuracy
      • BAFF/Blys: 101%
      • BRAK/CSCL14: 101%
      • CCL28: 91%
      • CXCL16: 103%
      • HCC-4/CCL16: 110%
      • HMGB1: 104%
      • IFNβ: 97%
      • IL-14: 104%
      • IL-19: 106%
      • IL-24: 99%
      • IL-28B: 101%
      • IL-32α: 89%
      • IL-34: 110%
      • IL-35: 95%
      • IL-36β: 98%
      • IL-37: 99%
      • IL-38: 104%
      • MIP-4/PARC/CCL18: 101%
      • MPIF-1/CL23: 102%
      • YKL40/CHI3L1: 100%
      Physicochemical Information
      Sensitivity
      • MPIF-1: 7.8 pg/mL
      • BRAK: 607 pg/mL
      • CXCL16: 4.7 pg/mL
      • HCC-4: 28.4 pg/mL
      • MIP-4/PARC: 6.6 pg/mL
      • IL-34: 121.5 pg/mL
      • IL-24: 0.8 ng/mL
      • IL-35: 0.8 ng/mL
      • IL-37/IL-1F7: 5.2 pg/mL
      • IL-19: 248.2 pg/mL
      • CCL28: 100.8 pg/mL
      • HMGB1/HMG1: 10.5 ng/mL
      • IFNβ: 375.6 pg/mL
      • IL-38: 33.2 pg/mL
      • IL-28B/IFN-λ3: 17.2 pg/mL
      • BAFF/BLyS: 0.9 ng/mL
      • IL-14/α-Taxilin: 27.7 pg/mL
      • IL-36β: 3.5pg/mL
      • IL-32α: 32.1 pg/mL
      • YKL40/CHI3L1: 0.30 ng/mL
      Standard Curve Range
      • BAFF/Blys: 0.44-400 ng/mL
      • BRAK/CXCL14: 488-500,000 pg/mL
      • CCL28: 98-100,000 pg/mL
      • CXCL16: 3-3,000 pg/mL
      • HMGB1: 2.0-2,000 ng/mL
      • IFNβ: 488 – 500,000 pg/mL
      • IL-14: 24-25,000 pg/mL
      • IL-19: 244-250,000 pg/mL
      • IL-24: 0.7-750 ng/mL
      • IL-28B: 29-30,000 pg/mL
      • IL-32α: 39-40,000 pg/mL
      • IL-34: 98-100,000 pg/mL
      • IL-35: 0.8-800 ng/mL
      • IL-36β: 2- 2,000 pg/mL
      • IL-37: 5 – 5,000 pg/mL
      • IL-38: 49-50,000 pg/mL
      • MIP-4/PARC/CCL18: 5-5,000 pg/mL
      • MPIF-1/CL23: 10-10,000 pg/mL
      • YKL40/CHI3L1: 0.1-100 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      HCY4MG-64K-PX20 04061841042662

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