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IPFL00010 Immobilon-FL en PVDF, 0,45 µm, rouleau de 26,5 cm x 3,75 m

Western blotting, dot/slot

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Principales applications: Western Blotting, Dot Blot
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IPFL00010
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      Aperçu

      Tableau de caractéristiques principal

      Principales applications
      WB, DB
      Description
      RéférenceIPFL00010
      Nom de marque
      • Immobilon
      DescriptionImmobilon-FL en PVDF, 0,45 µm, rouleau de 26,5 cm x 3,75 m
      Informations généralesOverview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      Applications:
      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      Informations produit
      Méthode de détectionFluorescence Chimifluorescence Chromogénie Chimiluminescence 125I Fluorescence
      Colorants compatibles
      • Tous les colorants fluorescents
      • Bleu brillant de Coomassie
      • Amido black
      • Rouge Ponceau-S
      • CPTS
      • Colorant Sypro® Ruby
      Code du filtreIPFL
      Couleur du filtreBlanc
      Quality LevelMQ400
      Applications
      ApplicationWestern blotting, dot/slot
      Principales applications
      • Western Blotting
      • Dot Blot
      Informations biologiques
      Adsorption de BSA205 µg/cm²
      Adsorption d'IgG de chèvre300 µg/cm²
      Adsorption d'insuline155 µg/cm²
      MouillabilitéHydrophobe
      Informations physico-chimiques
      Dimension de pores0.45 µm
      Dimensions
      Surface du filtreUnie
      Dimensions du filtre27 cm x 3.75 m
      Informations sur les matériaux
      Chimie
      • PVDF hydrophobe
      Informations sur l'emballage
      Quantité1
      Global Trade Item Number
      Référence GTIN
      IPFL00010 04053252669439