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GF446-50UG HumanKine® Thermostable bFGF,
Human Recombinant

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GF446-50UG
50 µg  
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      Tableau de caractéristiques principal

      SourcePurity
      HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.Product is endotoxin free (<0.5 ng/μg of protein (<5 EU/μg) and greater than 95% pure by SDS-PAGE.
      Description
      Catalogue NumberGF446-50UG
      Trade Name
      • HumanKine®
      DescriptionHumanKine® Thermostable bFGF,
      Human Recombinant
      Alternate Names
      • FGF2
      • prostatropin
      • Prostatropin
      • HBGF-2
      • BFGF
      Background InformationThe HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.

      Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.
      Product Information
      PresentationLyophilized from a 0.2 μm filtered solution of 1X PBS.
      Quality LevelMQ100
      Applications
      ApplicationEngineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
      Application NotesHumanKine products are Xeno-Free
      Biological Information
      PurityProduct is endotoxin free (<0.5 ng/μg of protein (<5 EU/μg) and greater than 95% pure by SDS-PAGE.
      SourceHumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
      UniProt Number
      Product Usage Statements
      Quality AssuranceBioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. EC50 is typically ≤0.5 ng/mL.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Référence GTIN
      GF446-50UG 04053252975950