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SCC249 Hep-G2/2.2.15 Human Hepatoblastoma Cell Line

The Hep-G2/2.2.15 human hepatoblastoma cell line is a suitable model for research into many aspects of HBV-associated liver cancer.

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SCC249
≥1X10⁶ cells/vial   
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      Replacement Information
      Description
      Catalogue NumberSCC249
      DescriptionHep-G2/2.2.15 Human Hepatoblastoma Cell Line
      Alternate Names
      • Hep-G2/2215
      • HepG2-2.2.15
      • HepG2 2.2.15
      • HepG2.2.15
      Background InformationHepatocellular carcinoma is the primary malignancy of the liver and a leading cause of cancer-related death in the world. The etiology of hepatocellular carcinoma is closely linked to infection with hepatitis B virus (1). Because of the causative role of HBV in liver cancers, cellular models of HBV-infected hepatocellular carcinomas have been developed for understanding the progression and fundamental biology of liver cancers.

      The Hep-G2/2.2.15 human hepatoblastoma cell line, a subclone of HepG2 human hepatoblastoma that stably expresses the hepatitis B virus, is one of the most widely used models for HBV-associated liver disease. Hep-G2/2.2.15 cells possess lower proliferation and invasion activity than parental HepG2 cells and have low tumorigenicity in nude mice (2). Like parental HepG2 cells, Hep-G2/2.2.15 cells express the microtubule-associated marker doublecortin (DCX).

      Source:
      Hep-G2/2.2.15 was produced from Hep-G2 cells stably transformed with the hepatitis-B virus (HBV) genome. Hep-G2/2.2.15 cells are resistant to G418 and contain two head-to-tail dimers of the HBV type D genome (3). The parental HepG2 cell line was isolated from hepatoblastoma tissue of a 15-year-old male patient (4).

      References:
      1. World J Gastroenterol. 2007; 13(32): 4295-42305.
      2. World J Gastroenterol. 2011; 17(9): 1152-1159.
      3. Proc Natl Acad Sci USA. 1987; 84(4): 1005-1009.
      4. Nature. 1979; 282(5739): 615-616.
      References
      Product Information
      Applications
      ApplicationThe Hep-G2/2.2.15 human hepatoblastoma cell line is a suitable model for research into many aspects of HBV-associated liver cancer.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Hepatocyte Cells
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells tested positive for Hepatitis B virus (HBV) and negative for all other infectious diseases in the Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCC249 04061841079842