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SCR077 Fluorescent Mouse ES/iPS Cell Characterization Kit

This Fluorescent Mouse ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse Embryonic stem & induced pluripotent Stem cells.

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Key Applications: Immunocytochemistry
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SCR077
1 Kit  
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      Key Applications
      ICC
      Description
      Catalogue NumberSCR077
      DescriptionFluorescent Mouse ES/iPS Cell Characterization Kit
      Background InformationMouse embryonic stem (mES) cells are pluripotent cells derived from the inner cell mass of pre-implantation blastocysts. Mouse induced pluripotent (miPS) cells are pluripotent cells generated by reprogramming mouse somatic cells using four transcription factors, Oct-4, Klf-4, Sox-2, and c-Myc, or their variants. Both mESC and miPSC can self-renew and have the ability to generate all three germ layers: ectoderm, mesoderm, and endoderm. In vitro, mESC/iPSC are normally maintained and propagated on mouse fibroblast feeders or in feeder-free conditions in media containing LIF/ESGRO. However, spontaneous differentiation may occur in subpopulations of cells. Several pluripotent markers are commonly used to distinguish pluripotent mESC/iPSC from differentiated cells.

      EMD Millipore’s Fluorescent Mouse ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse ES/iPS cells. Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and DPPA-2 and cell surface epitope SSEA-1 to enable rapid immunocytochemical marker analysis. The Dapi nuclear dye is conveniently included to aid in cell quantification. While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics.
      References
      Product Information
      Components
      • 1. Fast Red Violet solution (Part No. 90239): One 15 mL bottle.
      • 2. Napthol AS-BI phosphate solution (Part No. 90234). One 15 mL bottle.
      • 3. Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor® 488 conjugate (Part No. MAB4419A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
      • 4. Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate (Part No. MAB4423C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
      • 5. Mouse anti-DPPA-2, clone 6C1.2, Alexa Fluor® 488 conjugate (Part No. MAB4356A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
      • 5. Mouse anti-SSEA-1, clone MC-480, Cy3 conjugate (Part No. MAB4301C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
      • 8. DAPI, 100 µL (Part No. 90229). One vial containing 100 µL volume.
      PresentationPurified conjugated mouse monoclonal antibodies are stored in PBS with 0.1% sodium azide and 15mg/ml BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Fluorescent Mouse ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse Embryonic stem & induced pluripotent Stem cells.
      Key Applications
      • Immunocytochemistry
      Biological Information
      Cross ReactivityNone
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceConjugated antibodies and alkaline phosphatase reagents have been validated on mouse ES and STEMCCA derived mouse ips cells.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C until expiration date of kit. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCR077 04053252551901