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341631 Fibronectin, Bovine Plasma

Fibronectin, Bovine Plasma : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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341631
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341631-1MG
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      		 Ampoule plast. 1 mg
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      341631-5MG
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          		 Flacon en verre 5 mg
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          Description
          OverviewNative fibronectin purified from pooled bovine plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
          Catalogue Number341631
          Brand Family Calbiochem®
          References
          Product Information
          CAS number86088-83-7
          FormLiquid
          FormulationIn 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.
          Quality LevelMQ100
          Applications
          Biological Information
          Puritysingle band by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Référence GTIN
          341631-1MG 04055977194883
          341631-5MG 04055977194906

          Documentation

          Fibronectin, Bovine Plasma Certificats d'analyse

          TitreNuméro de lot
          341631
          Fiche technique

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-December-2010 RFH
          DescriptionNative fibronectin purified from pooled bovine plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
          FormLiquid
          FormulationIn 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          Protocol for Coating of Tissue Culture Plates with Fibronectin

          This protocol is provided only as a guideline; optimal conditions should be determined as needed. This procedure is based on the use of 21 cm2 dishes and 1 mg fibronectin, which is a sufficient quantity to coat 10 dishes at 5 µg/cm2.

          1. Thaw the fibronectin by placing the vial in a 37°C water bath until it is completely thawed. Be VERY careful during the thawing process. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.

          2. After thawing, bring the solution to a final volume of 20 ml with sterile, serum-free medium that has been pre-heated to 37°C. This yields a fibronectin solution of 50 µg/ml.

          3. Add 2 ml to each of 10 tissue culture dishes and swirl gently to completely coat the entrie growth surface.

          4. Incubate the dishes at room temperature for ~45 min to permit binding of the fibronectin to the growth surfaces.

          5. Tilt each dish and remove the fibronecting using a sterile pipette. Do not permit the pipette tip to disturb the growth surface.

          6. Add the cell suspension in medium directly to the fibronectin-coated dishes and incubate under conditions appropriate to the cells.
          CAS number86088-83-7
          Puritysingle band by SDS-PAGE
          Storage Avoid freeze/thaw
          -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          Merck USA index14, 4075