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ES005-10ML DAB 50x Concentrate, Brown, Horseradish Peroxidase Substrate (insoluble in alcohol)

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ES005-10ML
10 mL  
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      Tableau de caractéristiques principal

      Key Applications
      WB, IHC
      Description
      Catalogue NumberES005-10ML
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDAB 50x Concentrate, Brown, Horseradish Peroxidase Substrate (insoluble in alcohol)
      OverviewSince first introduced by Graham and Karnovsky (1966), numerous procedures for its use in histochemistry, immunohistochemistry, Western and dot blots have been described (Lewis & Knight, 1977; Larsson, 1988; Gallyas et al., 1982). In the presence of horseradish peroxidase and hydrogen peroxide, DAB oxidatively polymerizes to an insoluble brown polymer. The color can be modified and intensified by treatment with metal salts of silver, copper, nickel, cobalt and osmium (Hsu & Soban, 1982). DAB is a suspected carcinogen and must be handled with caution. CHEMICON supplies DAB as a stable liquid concentrate eliminating the necessity of handling a potentially hazardous material. The stabilization system is unique and prevents formation of partially oxidized DAB excluding nonspecific binding to other heme containing proteins so often observed with powdered DAB preparations. The concentrate can be diluted in appropriate peroxide containing buffers providing the researcher with the capability of formulating any of the numerous published DAB reaction systems. The most common procedure suggests diluting the DAB 1:50 with Chemicon Catalog Number ES010 (Tris-HCl buffer, pH 7.6), and adding H(2)O(2), to a final concentration of 0.01%. Incubation times range from 5-15 minutes. Prolonged reaction time will result in non-specific staining of other heme proteins.
      Product Information
      PresentationDAB (50X) concentrate in proprietary solvents and stabilizers.
      Color CodeBlack
      Applications
      Application10 ml DAB 50x Concentrate, Brown, Horseradish Peroxidase Substrate (insoluble in alcohol) for Western Blotting and Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for up to 6 months after date of receipt. DAB is a suspected carcinogen.
      Packaging Information
      Material Size10 mL
      Global Trade Item Number
      Référence GTIN
      ES005-10ML 04053252410536