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234155 Collagenase, Type II, Clostridium histolyticum

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      Aperçu

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Crude collagenase preparation that contains higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required.
      Catalogue Number234155
      Brand Family Calbiochem®
      References
      ReferencesKeller, S., and Mandl, I. 1963. Arch. Biochem. Biophys. 101, 81.
      Product Information
      CAS number9001-12-1
      Activity≥200 collagenase units/mg dry weight
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.
      EC number3.4.24.3
      FormLyophilized
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      ContaminantsNon-specific proteolytic activity: ≥50 fluorescence units/mg
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 42/43-36/37/38

      May cause sensitization by inhalation and skin contact.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 22-24-37

      Do not breathe dust.
      Avoid contact with skin.
      Wear suitable gloves.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsUnstable in solution; reconstitute just prior to use.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      234155 0

      Documentation

      Collagenase, Type II, Clostridium histolyticum Certificats d'analyse

      TitreNuméro de lot
      234155

      Références bibliographiques

      Aperçu de la référence bibliographique
      Keller, S., and Mandl, I. 1963. Arch. Biochem. Biophys. 101, 81.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-August-2010 RFH
      DescriptionCrude collagenase solid prepared to contain higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required. Has an optimal pH of 7.0-8.0.
      FormLyophilized
      CAS number9001-12-1
      EC number3.4.24.3
      ContaminantsNon-specific proteolytic activity: ≥50 fluorescence units/mg
      Activity≥200 collagenase units/mg dry weight
      Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.
      SolubilityAqueous Buffers
      Storage Protect from moisture
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsUnstable in solution; reconstitute just prior to use.
      Toxicity Harmful
      Merck USA index14, 2481
      ReferencesKeller, S., and Mandl, I. 1963. Arch. Biochem. Biophys. 101, 81.