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SCT265 BioTracker™ Green Exosome Membrane Labeling Kit

This live cell probe fluoresces in the green spectral range when it binds to the lipid bilayer in exosome membranes.

SCT265
5 reactions  
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      Replacement Information
      Description
      Catalogue NumberSCT265
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      DescriptionBioTracker™ Green Exosome Membrane Labeling Kit
      OverviewThe BioTracker™ exosome membrane labeling probe series can be used for staining of the membranes of purified exosomes, which in turn permits imaging of labeled exosomes taken up by cells.

      SCT265 does not cause aggregation and has little effect on the properties of exosomes, permitting accurate observation of exosome dynamics.

      BioTracker™ exosome membrane labeling probes overcome issues with existing labeling dyes, including:
      1) self-particle formation
      2) an increase in size of the exosomes after labeling

      Three different fluorescence colors---green (SCT265), red (SCT266), and far red (SCT267)--- enable application in multiplexing experiments, such as observation of exosome localization in organelles.

      Add 10 uL DMSO to tube of green, red, or far red exosome membrane dye to create stock solution.



      SPECTRAL PROPERTIES:
      Ex 488nm/Em 490-540nm

      References:
      Kawata R, Oda S, Koya Y, Kajiyama H, Yokoi T. 2020. Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production. Toxicology. 443:152544.
      Alternate Names
      • green exosome probe
      • exosome labeling kit
      • exosome membrane probe
      • green exosome dye
      Background InformationExosomes are a form of extracellular vesicle (EV), which can contribute to intercellular communication. To elucidate such communication, labeling techniques based on fluorescent dyes have been developed. Fluorescent dyes that label the cellular membrane are commonly used for exosome labeling because the lipid bilayer in exosomes is a suitable labeling target.
      References
      Product Information
      FormatLyophilized solid
      PresentationLyophilized solid
      Quality LevelMQ100
      Applications
      ApplicationThis live cell probe fluoresces in the green spectral range when it binds to the lipid bilayer in exosome membranes.
      Key Applications
      • Cell Based Assays
      • Cell Culture
      • Flow Cytometry
      • Fluorescence
      • Imaging Flow Cytometry
      • Pharmaceutical
      Application NotesThis live culture probe for the detection of exosomes labels the lipid bilayers of exosomal membranes, enabling observation of the uptake of exosomes by cells. Exosomes should be purified before labeling.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker™ Green Exosome Membrane Labeling Kit at 2 - 8 °C, protected from light.
      After resuspending in DMSO, store stock solution at -20 °C, protected from light.
      Packaging Information
      Material Size5 reactions
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCT265 04065269218197

      Documentation

      Produits & Applications associés