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SCT271 BioTracker™ Green Endocytosis Live Cell Probe

pH-dependent fluorescent live cell probe for visualization of endocytosis from the early endosome stage.
Fluorescent Dye-Dextran Conjugates or membrane staining reagents are used to visualize endocytosis. However, they have limitations in observing the dynamics of endosomes in live cells in terms of precision of staining or retentivity of reagent. SCT271 is a different kind of reagent that overcomes these limitations.

NouveauSCT271
40 µL  
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      Replacement Information
      Description
      Catalogue NumberSCT271
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      DescriptionBioTracker™ Green Endocytosis Live Cell Probe
      OverviewBioTracker™ Green Endocytosis Live Cell Probe is a pH-dependent fluorescent dye that localizes to the vesicle membrane. Visualization of endocytosis using SCT271 is a more direct method than fluorescent analogs, and allows visualization of endocytosis.

      *Precise visualization of endocytosis
      *Track endocytosis using live cells
      *High responsiveness to pH change


      SPECTRAL PROPERTIES
      λex: 386 nm
      λem: 522 nm

      Suggested observation conditions:
      λex: 350 - 410 nm
      λem: 500 - 560 nm
      Alternate Names
      • endocytosis probe
      • green endocytosis probe
      • endocytosis live cell probe
      • endocytosis fluorescent probe
      Background InformationEndocytosis is a cellular process that describes macromolecules being taken up through the plasma membrane-derived endosome vesicle. The endocytic pathway contributes to maintaining intracellular homeostasis by transporting various nutrients into the cell and shuttling unneeded components to the lysosome, which functions as a waste disposal system. It has been demonstrated that disruption of endocytosis is related to some neurodegenerative dysfunction and immune diseases. Investigation of the endocytic pathway is therefore of increasing interest to cell biologists and in diverse disease disciplines.
      References
      Product Information
      FormatLiquid Reagent
      Presentationgreen liquid
      Quality LevelMQ100
      Applications
      ApplicationpH-dependent fluorescent live cell probe for visualization of endocytosis from the early endosome stage.
      Fluorescent Dye-Dextran Conjugates or membrane staining reagents are used to visualize endocytosis. However, they have limitations in observing the dynamics of endosomes in live cells in terms of precision of staining or retentivity of reagent. SCT271 is a different kind of reagent that overcomes these limitations.
      Key Applications
      • Cell Based Assays
      • Fluorescence
      Application NotesThis live cell probe is a non membrane-permeable molecule that fluoresces on internalization into mammalian cells,enabling direct observation of the membrane of internalized vesicles.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C, protected from light.
      Packaging Information
      Material Size40 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCT271 04065270661326