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200497 Betacellulin, Mouse, Recombinant

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200497
  
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.



      Recombinant, mouse betacellulin (BTC) cinsisting of amino acids 32-118 and expressed in E. coli. Mouse BTC is 93% identical to the amino acid sequence of rat and 79% with that of human. BTC, a heparin-binding protein, is expressed in tissues such as uterus, liver, pancreas, and kidney and promotes pancreatic beta-cell growth and differentiation. Also known as a potent mitogen for retinal pigment epithelial cells, fibroblasts, and vascular smooth muscle cells.

      Catalogue Number200497
      Brand Family Calbiochem®
      References
      ReferencesBastian, S.E., et al. 2001. J. Endocrinol. 168, 203.
      Li, L., et al. 2001. Endocrinology 142, 5379.
      Dunbar, A.J. and C. Goddard. 2000. Int. J. Biochem. Cell Biol. 32, B805.
      Tada, H., et al. 1999. J. Cell Biochem. 72, 423.
      Pathak, B.G., et al. 1995. Genomics 28, 116.
      Shing, Y., et al. 1993. Science 259, 1604.
      Product Information
      FormLiquid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 0.15-0.6 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 Eu/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      200497 0

      Documentation

      Betacellulin, Mouse, Recombinant FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Betacellulin, Mouse, Recombinant Certificats d'analyse

      TitreNuméro de lot
      200497

      Références bibliographiques

      Aperçu de la référence bibliographique
      Bastian, S.E., et al. 2001. J. Endocrinol. 168, 203.
      Li, L., et al. 2001. Endocrinology 142, 5379.
      Dunbar, A.J. and C. Goddard. 2000. Int. J. Biochem. Cell Biol. 32, B805.
      Tada, H., et al. 1999. J. Cell Biochem. 72, 423.
      Pathak, B.G., et al. 1995. Genomics 28, 116.
      Shing, Y., et al. 1993. Science 259, 1604.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-Sepmtember-2011 RFH
      DescriptionRecombinant, mouse betacellulin (BTC) cinsisting of amino acids 32-118 and expressed in E. coli. Mouse BTC is 93% identical to the amino acid sequence of rat and 79% with that of human. BTC, a heparin-binding protein, is expressed in tissues such as uterus, liver, pancreas, and kidney and promotes pancreatic beta-cell growth and differentiation. Also known as a potent mitogen for retinal pigment epithelial cells, fibroblasts, and vascular smooth muscle cells.
      FormLiquid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 Eu/µg protein
      Biological activityED₅₀ = 0.15-0.6 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
      SolubilityReconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesBastian, S.E., et al. 2001. J. Endocrinol. 168, 203.
      Li, L., et al. 2001. Endocrinology 142, 5379.
      Dunbar, A.J. and C. Goddard. 2000. Int. J. Biochem. Cell Biol. 32, B805.
      Tada, H., et al. 1999. J. Cell Biochem. 72, 423.
      Pathak, B.G., et al. 1995. Genomics 28, 116.
      Shing, Y., et al. 1993. Science 259, 1604.