Millipore Sigma Vibrant Logo

69451 BL21(DE3)pLysS Competent Cells - Novagen

BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

BL21(DE3)pLysS Competent Cells - Novagen : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
View Products on Sigmaaldrich.com
69451
Voir les Prix & la Disponibilité

Aperçu

Replacement Information

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
69451-3
Récupération des données relatives à la disponibilité...
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      		 Flacon en verre 0.4 ml
      Prix en cours de récupération
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      —
      Demander le prix
      		 —
      69451-4
      Récupération des données relatives à la disponibilité...
      —
      Disponibilité limitée Disponibilité limitée
      En stock 
      Interrompu(e)
      Disponible en quantités limitées
      Disponibilité à confirmer
        Pour le restant : Nous vous tiendrons informé
          Pour le restant : Nous vous tiendrons informé
          Nous vous tiendrons informé
          Contacter le Service Clients
          Contact Customer Service

          		 Flacon en verre 1 ml
          Prix en cours de récupération
          Le prix n'a pas pu être récupéré
          La quantité minimale doit être un multiple de
          Maximum Quantity is
          À la validation de la commande Plus d'informations
          Vous avez sauvegardé ()
           
          —
          Demander le prix
          		 —
          Description
          OverviewBL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

          DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

          pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
          Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)


          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number69451
          Brand Family Novagen®
          References
          Product Information
          Components
          Guaranteed efficiency> 2 x 10⁷ cfu/μg
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Référence GTIN
          69451-3 04055977274356
          69451-4 04055977274363

          Documentation

          BL21(DE3)pLysS Competent Cells - Novagen FDS

          Titre

          Fiche de données de sécurité des matériaux (FDS) 

          BL21(DE3)pLysS Competent Cells - Novagen Certificats d'analyse

          TitreNuméro de lot
          69451

          Brochure

          Titre
          Competent Cell Brochure

          Citations

          Titre
        • Alvarez, V.E., et al. 2008. Journal of Biological Chemistry 283, 3454.
        • Khan, M.A., et al. 2008. Journal of Biological Chemistry 283, 1340.
        • Luttge, B.G., et al. 2008. Journal of Virology 82, 2106.
        • Mittra, B., et al. 2008. Journal of Biological Chemistry 283, 3161.
        • Geisler, D.A., et al. 2007. Journal of Biological Chemistry 282, 28455.
        • Wu, A.T.H., et al. 2007. Journal of Biological Chemistry 282, 12164.
        • Yu, Z., et al. 2007. Nucleic Acids Research 35, 2107.
        • Gong, B., et al. 2006. Cell 126, 775.
        • Annika Grabbe and Jurgen Wienands. 2006. Blood 108, 3761.
        • Grobe, A.C., et al. 2006. American Journal of Physiology: Lung 290, L1069.
        • LeFebvre, A.K., et al. 2006. Journal of Biological Chemistry 281, 22917.
        • Schmelz, E.A., et al. 2006. Proceedings of the National Academy of Sciences (USA) 103, 8894.
        • Ellen J. Tisdale and Cristina R. Artalejo. 2006. Journal of Biological Chemistry 281, 8436.
        • M. Wang and K. A. Hudak. 2006. Nucleic Acids Research 34, 1174.
        • Ashida, H., et al. 2005. Plant and Cell Physiology 46, 557.
        • Attinger, A., et al. 2005. Journal of Immunology 174, 3501.
        • Banci, L., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 3994.
        • Hsiu-Hui Chen, H-H., et al. 2005. Blood 105, 3902.
        • Cherny, I.,et al. 2005. Journal of Biological Chemistry 280, 30063.
        • Chiarelli, L.R., et al. 2005. Blood 105, 3340.
        • Dattelbaum, J.D., et al. 2005. Protein Science 14, 284.
        • Dufour, E.K., et al. 2005. Protein Science 14, 303.
        • Flint, J., et al. 2005. Journal of Biological Chemistry 280, 23718.
        • Funa, N., et al. 2005. Journal of Biological Chemistry 280, 14514.
        • Hamburger, A.E., et al. 2005. Journal of Biological Chemistry 280, 12567.
        • Handa, N., et al. 2005. Protein Science 14, 1004.
        • Herron, B.J., et al. 2005. Human Molecular Genetics 14, 667.
        • Islam, M.N., et al. 2005. Protein Engineering Design and Selection 18, 71.
        • Kawakami, H., et al. 2005. Journal of Biological Chemistry 280, 27420.
        • Han Ie Kim and Seung-Taek Lee. 2005. Journal of Biological Chemistry 280, 28973.
        • Kim, S.H., et al. 2005. Molecular and Cellular Proteomics 4, 267.
        • Korneeva, N.L., et al. 2005. Journal of Biological Chemistry 280, 1872.
        • Kotoshiba, S., et al. 2005. Journal of Biological Chemistry 280, 17694.
        • Kwade, Z., et al. 2005. Journal of Biological Chemistry 280, 17512.
        • Li, C., et al. 2005. Journal of Biological Chemistry 280, 24864.
        • Li, H., et al. 2005. Protein Science 14, 3025.
        • Madsen, K.L., et al. 2005. Journal of Biological Chemistry 280, 20539.
        • Mori, S., et al. 2005. Journal of Biological Chemistry 280, 24104.
        • Mukhopadhyay, S., et al. 2005. Journal of Biological Chemistry 280, 538.
        • Nowotny, M., et al. 2005. Cell 121, 1005.
        • Ruthenburg, A.J., et al. 2005. Journal of Biological Chemistry 280, 26177.
        • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
        • Tóth, K.F., et al. 2005 Journal of Biological Chemistry 280, 15690.
        • Takahashi, H., et al. 2005. Journal of Biological Chemistry 280, 5611.
        • Tang, Q-Q., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 9766.
        • Yamauchi, J., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 5198.
        • Bühling, F., et al. 2004. Clin. Diagn. Lab. Immunol. 11, 131.
        • Bittker, J.A., et al. 2004. Procedings of the National Academy of Science 101, 7011.
        • Yang Chao and Dax Fu. 2004. Journal of Biological Chemistry 279, 17173.
        • Tran C. Dong and Simon M. Cutting. 2004. Journal of Biological Chemistry 279, 43468.
        • Ferreira, V., et al. 2004. The Journal of Immunology 172, 3042.
        • Gu, D., et al. 2004. Journal of Biological Chemistry 279, 31171.
        • Jeong, S.Y., et al. 2004. Planta 219, 298.
        • Richard, C.L., et al. 2004. Journal of Bacteriology 186, 740.
        • Sanfelice, D., et al. 2004. Journal of Biomolecular NMR 29, 449.
        • Tisdale, E.J., et al. 2004. Journal of Biological Chemistry 279, 54046.
        • Xiao, K., et al. 2004. Journal of Biological Chemistry 279, 55744.
        • Zhang, Y.X., et al. 2004. Cell Research 14, 208.
        • Albers, M., et al. 2003. RNA 9, 138.
        • BMazumder, B., et al. 2003. Cell 115, 187.
        • Premkumar, L., et al. 2003. Protein Expression and Purification 28, 151.
        • Huhtinen, K., et al. 2002. Journal of Biological Chemistry 277, 3424.
        • Renshaw, P.R., et al. 2002. Journal of Biological Chemistry 277, 21598.
        • Chazalet, V., et al. 2001. Journal of Bacteriology 183, 7067.
        • Glick, E., et al. 2001. European Molecular Biology Organization Journal 20, 7303.
        • Brandt, J.J., et al. 2000. Protein Expression and Purification 20, 300.
        • Cabral, J.H.M., et al. 1998. Cell 95, 649.
        • A.K. Gombert and B.V. Kilikan. 1998. Journal of Biotechnology 60, 47.
        • Vinkemeir, U., et al. 1998. Science 279, 1048.
        • Black, S.M., et al. 1997. Journal of Clinical Investigation 100, 1448.
        • Bochkarev, A., et al. 1997. Nature 385, 176.
        • Shanley, T.P., et al. 1997.

          • Protocoles Utilisateur

          Titre
          TB009 Competent Cells
          TB053 Academic and Non-profit Laboratory Assurance Letter

          Produits & Applications associés

          Related Products

          Référence Description  
          69449 BL21 Competent Cells - Novagen Prix & Disponibilité
          69450 BL21(DE3) Competent Cells - Novagen Prix & Disponibilité
          70232 BL21 Competent Cell Set - Novagen Prix & Disponibilité

          Catégories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression