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MAB3806-C Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free is an antibody against phospho-ATM for use in Immunocytochemistry, Immunoprecipitation, ELISA, Western Blotting.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: ATM, AT UniProt Number: Q13315
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MAB3806-C
100 μg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IP, ELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3806-C
      DescriptionAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free
      Alternate Names
      • Serine-protein kinase ATM, Ser1981 phosphorylated
      • Ataxia telangiectasia mutated, Ser1981 phosphorylated
      • A-T mutated, Ser1981 phosphorylated
      Background InformationSerine-protein kinase ATM (EC 2.7.11.1; UniProt Q13315; also known as A-T mutated, Ataxia telangiectasia mutated) is encoded by the ATM (also known as AT) gene (Gene ID 472) in human. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). Known ATM substrates include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The S/TQ sequence constitutes the essential substrates phosphorylation site motif. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate phosphorylation, while positively charged residues surrounding the S/TQ are negative determinants. The complex phenotypes of cells derived from patients with AT suggests that ATM has additional cellular substrates. ATM is present as an inactive homodimer or multimer prior to activation. DNA double-stranded breaks induced by ionizing radiation (IR) cause rapid ATM autophosphorylation at Ser1981 in human or the Ser1987 equivalent in mouse.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free is an antibody against phospho-ATM for use in Immunocytochemistry, Immunoprecipitation, ELISA, Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • ELISA
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot detected Ser1981-phosphorylated ATM interaction with endogenous EphA5 by co-immunoprecipitation using chromatin-enriched protein fractions of irradiated NCI-H460 human lung cancer cells (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
      ELISA Analysis: a representative lot was employed as the capture antibody for the detection of interaction between EphA5 and Ser1981-phosphorylated ATM. ATM pSer1981 captured from NCI-H460 human lung cancer cell extracts interacted with recombinant EphA5 cytoplasmic domain, but not with EphA5 extracellular domain or kinase domain (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
      Immunocytochemistry Analysis: A representative lot detected radiation-/IR-induced upregulation of nuclear ATM pSer1981 foci by fluorescent immunocytochemistry staining of methanol-fixed NCI-H460 human lung cancer cells (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
      Immunocytochemistry Analysis: A representative lot detected radiation-/IR-induced ATM Ser1981 phosphorylation by fluorescent immunocytochemistry staining of 1.2% formaldehyde-fixed, 0.1% Triton X-100-permeabilized HT29 cells (Bardelle, C., and Boros, J. (2012). J. Biomol. Screen. 17(7):912-920).
      Immunocytochemistry Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation by fluorescent immunocytochemistry staining of methanol/acetone-fixed primary human foreskin fibroblasts (HFFs) (Bakkenist, C.J., et al. (2004). Cancer Res. 64(11):3748-3752).
      Western Blotting Analysis: A representative lot detected epirubicin-induced ATM Ser1981 (Ser1987 in mouse) phosphorylation in mouse embryonic fibroblasts (MEFs) and human MCF-7 cells (Khongkow, P., et al. (2014). Oncogene. 33(32): 4144-4155).
      Western Blotting Analysis: A representative lot detected camptothethin-induced ATM Ser1987 (Ser1981 in human) phosphorylation in primary mouse cortical neurons (Brochier, C., et al. (2013). J. Neurosci. 33(20): 8621-8632).
      Western Blotting Analysis: A representative lot detected radiation-/IR-induced ATM Ser1981 phosphorylation in HT29, HeLa, and HEK293T cells (Bardelle, C., and Boros, J. (2012). J. Biomol. Screen. 17(7):912-920).
      Western Blotting Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation in U2OS cells by Western blotting using whole cell lyates or B56γ immunoprecipitate (Shouse, G.P., et al. (2011). Oncogene. 30(35):3755-3765).
      Western Blotting Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation in cultured primary human foreskin fibroblasts (HFFs), as well as passage-dependent basal ATM Ser1981 phosphorylation levels in HFFs (Bakkenist, C.J., et al. (2004). Cancer Res. 64(11):3748-3752).
      Biological Information
      ImmunogenSynthetic peptide corresponding to a.a. 1974-1988 of human ATM with phosphorylated Ser1981 (SLAFEEG[pS]QSTTISS).
      EpitopepSer1981.
      Clone10H11.E12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityReacts with ATM Kinase phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ATM
      • AT
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight350.7 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected Camptothecin (Cat. No. 208925) treatment-induced increase of nuclear ATM pSer1981 foci in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MAB3806-C 04055977334210

      Documentation

      Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free Certificats d'analyse

      TitreNuméro de lot
      Anti-phospho-ATM (Ser1981), -Q2669086 Q2669086
      Anti-phospho-ATM (Ser1981), clone10H11.E12, Ascites Free - 3188587 3188587
      Anti-phospho-ATM (Ser1981), clone10H11.E12, Ascites Free - 3457651 3457651
      Anti-phospho-ATM (Ser1981), clone10H11.E12, Ascites Free - 3884321 3884321
      Anti-phospho-ATM (Ser1981), clone10H11.E12, Ascites Free - 3923520 3923520
      Anti-phospho-ATM (Ser1981), clone10H11.E12, Ascites Free - 3995075 3995075
      Anti-phospho-ATM (Ser1981), clone10H11.E12, Ascites Free - 4082552 4082552

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies