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PC347 Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb

This Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb is validated for use in Frozen Sections, Immunoblotting for the detection of Serotonin 3 (5-HT₃) Receptor (444-457).

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PC347
  
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      Aperçu

      Tableau de caractéristiques principal

      Species ReactivityHostAntibody Type
      M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the serotonin 3 (5-HT3) receptor in mouse and rat brain tissue.
      Catalogue NumberPC347
      Brand Family Calbiochem®
      Application Data
      Detection of human Serotonin 5-HT3 Receptor by staining frozen sections. Samples: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Serotonin (5-HT3) Receptor (444-457) Rabbit pAb (Cat. No. PC347) (5 µg/ml). Detection: DAB.
      References
      ReferencesMorales, M., et al. 1998. J. Comp. Neurol. 385, 385.
      Morales, M., et al. 1996. Brain Res. 731, 199.
      Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
      Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
      Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
      Maricq, A.V., et al. 1991. Science 254, 432.
      Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
      Derkach, V., et al. 1989. Nature 33, 706.
      Product Information
      FormLiquid
      Formulation25 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin
      Positive controlMouse or rat brain tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Not Western Blot
      Application NotesFrozen Sections (2-5 µg/ml)
      Immunoblotting (not recommended)
      Application CommentsThis antibody is not recommended for immunoblotting using total mouse or rat brain extract. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M. Unconjugated serotonin 5-HT3 receptor control peptide has been included for use as an internal control for antibody specificity. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (SLEKRDEMREVARD) corresponding to amino acids 444-457 of rat serotonin (5-HT₃) receptor, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Référence GTIN
      PC347 0