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AB3242 Anti-PGC-1 Antibody

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AB3242
100 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, ICC, IF, IHC, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3242
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PGC-1 Antibody
      Alternate Names
      • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
      • Ligand effect modulator 6
      • PGC-1-alpha
      • PPAR-gamma coactivator 1-alpha
      • PPARGC-1-alpha
      Background InformationPGC-1 is a transcription factor that determines whether preadipocytes will differentiate into brown or white fat cells. It interacts with PPARgamma to promote the expression of a cassette of genes which define the brown adipocyte phenotype.
      Product Information
      FormatAffinity Purified
      Control
      • BAT nuclear prep
      PresentationPurified rabbit polyclonal antibody in buffer containing 20 mM PBS and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PGC-1 Antibody is a rabbit olyclponal antibody for the detection of PGC-1 also known as PPARgamma Coactivator 1-alpha & has been validated in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesELISA Analysis: A 1:75,000 dilution of a previous lot was used in ELISA.

      Immunocytochemistry Analysis: A 1:300 dilution of a previous lot was used in Immunocytochemistry.

      Immunofluorescence Analysis: Representative lots detected PGC-1α in frozen mouse and rat muscle tissue sections (Gouspillou, G., et al. (2013). Longev. Healthspan. 2(1):13; Matsuura, T., et al. (2007). J. Orthop. Res. 25(11):1534-1540).

      Immunohistochemistry Analysis: A representative lot detected PGC-1α in free-floating mouse forebrain sections (Tallaksen-Greene, S.J., et al. (2014). J Neurosci. 34(47):15658-15668).

      Immunoprecipitation Analysis: Representative lots immunoprecipitated PGC-1α from mouse liver, skeletal muscle, and brown adipose tissue lysates (Boutant, M., et al. (2016). Cell Rep. 14(9):2068-2075; Park, R.D., et al. (2014). J. Exerc. Nutrition Biochem. 18(1):1-7; Kim, J.S., et al. (2014). J. Exerc. Nutrition Biochem. 18(3):259-266; Philip, A., et al. (2011). J. Biol. Chem. 286(35):30561-30570).

      Western Blotting Analysis: Representative lots detected similar PGC-1α in human, mouse, and rat skeletal muscle tissue, as well as in nuclear and mitochondrial preparations from rat brown adipose tissue (Suwa, M., et al. (2015). J. Sports Sci. Med. 14(3):548-555; Lee, I., et al. (2015). Front. Pharmacol. 6:43; Vincent, G., et al. (2015). Front Physiol. 6:51; Lombardi, A., et al. (2015). PLoS One. 10(2):e0116498; Gouspillou, G., et al. (2014). FASEB J. 28(4):1621-1633; Saleem, A., et al. (2014). Am. J. Physiol. Cell Physiol. 306(3):C241-249).
      Biological Information
      ImmunogenSynthetic peptide from amino acids 777-797 (C-terminus) of mouse PGC-1.
      EpitopeC-terminus
      HostRabbit
      SpecificityImmunogen peptide blocking prior to Western blotting completely abolished target band detection by this rabbit polyclonal antibody.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
      Gene Symbol
      • Ppargc1a
      • Pgc1
      • Pgc1a
      • Ppargc1
      Purification MethodAffinity purified
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis.
      SIZE: 797 amino acids; 90,588 Da
      SUBUNIT: Binds MYBBP1A, which inhibits transcriptional activation by this protein (By similarity). Interacts with LRPPRC. Homooligomer.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Brown adipose tissue, heart, kidney and brain.
      SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
      Molecular Weight~100 kDa observed. 91.03/89.64/91.71/77.14 kDa (human isoform 1/B4/B5/L-PGG-1alpha), 90.59 kDa (mouse isoform 1; PGC-1a1), 90.62 kDa (rat) calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse BAT extracts.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant mouse PGC-1, as well as endogenous PGC-1 in 15 µg of nuclear extract and whole cell lysate from mouse brown adipose tissue (BAT). Immunogen peptide blocking completely blocked target band detection.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -70ºC from date of receipt in undiluted aliquots. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Référence GTIN
      AB3242 04053252320866