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475923 Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb

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Synonymes: Anti-M1

475923
  
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      Aperçu

      Tableau de caractéristiques principal

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~120 kDa muscarinic M1 acetylcholine receptor protein in rat brain. Supplied with a control peptide.
      Catalogue Number475923
      Brand Family Calbiochem®
      SynonymsAnti-M1
      Application Data
      Detection of rat muscarinic M1 acetylcholine receptor by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the fusion protein antigen (lane 2). Primary antibody: Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb (Cat. No. 475923) (1:200). Detection: chemiluminescence.
      References
      ReferencesLevey, A.I., et al. 1991. J. Neurosci. 11, 3218.
      Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
      Smith. D.B., et al. 1988. Gene 67, 31.
      Allard, W.J., et al. 1987. Nucleic Acids Res. 15, 10604.
      Peralta, E.G., et al. 1987. EMBO J. 6, 3923.
      Product Information
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 60 µg lyophilized control protein.
      Positive controlM1-GST fusion protein (supplied) or rat brain
      Preservative≤0.1% sodium azide (antibody only)
      Applications
      Application ReferencesImmunohistochemistry, Immunoprecipitation Levey, A.I., et al. 1991. J. Neurosci. 11, 3218. Immunoprecipitation Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:200-1:300, chemiluminescence)
      Immunohistochemistry (see application references)
      Immunoprecipitation (see application references)
      Application CommentsThe epitope is specific for M1 and is not similar to any other known protein. To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. For use as a positive control in immunoblotting, use 10 ng/lane of the M1-GST fusion protein (minigel). For use as a negative control, preincubate 3-5 µg of M1-GST fusion protein with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 227-353 of the i3 intracellular loop of human M1 muscarinic acetylcholine receptor fused to GST
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 50 µl dH₂O to yield a stock concentration of 300 µg/ml. For long-term storage, aliquot the antibody and freeze (-20°); for short-term storage, refrigerate (4°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the M1-GST fusion protein with 100 µl H₂O, aliquot, and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Global Trade Item Number
      Référence GTIN
      475923 0