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MAB3747 Anti-Microphthalmia (Mi) Antibody, clone C5

Anti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, IHC, IHC(P), IP & WB.

Anti-Microphthalmia (Mi) Antibody, clone C5 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: LZMS, MCOPS1, MAA
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MAB3747
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MEMSA, IHC, IH(P), IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3747
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Microphthalmia (Mi) Antibody, clone C5
      Background InformationMiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3' half of Mi including most of the b-HLH-ZIP region. Osteoclasts from these animals lack Mi protein in contrast to wild-type rat, mouse, and human osteoclasts.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • 501 Mel human melanoma cells, wild-type human, rat, mouse osteoclast cells.
      PresentationPurified mouse monoclonal IgG1 in phosphate buffered saline with 0.08% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, IHC, IHC(P), IP & WB.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      A previous lot of this antibody was used in IP. (2 µg/mg of protein lysate)

      Gel supershift assays:
      A previous lot of this antibody was used in EMSA.

      Immunohistochemistry(paraffin):
      A previous lot of this antibody was used in IH on frozen and formalin/paraffin tissue sections.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
      CloneC5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityIn Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi). There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells andheart. It reacts with both melanocytic as well as the nonmelanocytic isoforms of Mi. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteExpected to react with human and rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • LZMS
      • MCOPS1
      • MAA
      Purification MethodProtein A Purfied
      Molecular Weight53-56 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected Mi on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MAB3747 04053252501678

      Documentation

      Anti-Microphthalmia (Mi) Antibody, clone C5 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Microphthalmia (Mi) Antibody, clone C5 Certificats d'analyse

      TitreNuméro de lot
      Anti-Microphthalmia (Mi), clone C5 3082557
      Anti-Microphthalmia (Mi), clone C5 - 2397033 2397033
      Anti-Microphthalmia (Mi), clone C5 - 2037527 2037527
      Anti-Microphthalmia (Mi), clone C5 - 2061433 2061433
      Anti-Microphthalmia (Mi), clone C5 - 2198942 2198942
      Anti-Microphthalmia (Mi), clone C5 - 2293865 2293865
      Anti-Microphthalmia (Mi), clone C5 - 2491103 2491103
      Anti-Microphthalmia (Mi), clone C5 - 3023021 3023021
      Anti-Microphthalmia (Mi), clone C5 - 3250325 3250325
      Anti-Microphthalmia (Mi), clone C5 - 3301944 3301944
      Show more

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Partially purified components of Nardostachys chinensis suppress melanin synthesis through ERK and Akt signaling pathway with cAMP down-regulation in B16F10 cells.
      Ji Yeon Jang,Ha Neui Kim,Yu Ri Kim,Woo Young Choi,Yung Hyun Choi,Hwa Kyoung Shin,Byung Tae Choi
      Journal of ethnopharmacology  137  2010

      Afficher le résumé       		21816215 21816215
      Inhibitory effects of 1-O-methyl-fructofuranose from Schisandra chinensis fruit on melanogenesis in B16F0 melanoma cells.
      Eun Young Oh,Ji Yeon Jang,Yung Hyun Choi,Young Whan Choi,Byung Tae Choi
      Journal of ethnopharmacology  132  2009

      Afficher le résumé       		20723590 20723590
      MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes.
      Hemesath, T J, et al.
      Nature, 391: 298-301 (1998)  1998

      Afficher le résumé       		9440696 9440696