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MABF1479 Anti-Ly-6G (mouse), clone RB6-8C5, biotin conjugate Antibody

This rat monoclonal Anti-Ly-6G (mouse), clone RB6-8C5, biotin conjugate, Cat. No. MABF1479 is validated for use in Flow Cytometry.

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Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Ly6g UniProt Number: P35461
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MABF1479
25 μg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCRAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF1479
      DescriptionAnti-Ly-6G (mouse), clone RB6-8C5, biotin conjugate Antibody
      Alternate Names
      • Lymphocyte antigen 6G (Ly-6G)
      • Ly-6G.1
      • Ly-6G
      Background InformationThe RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal Anti-Ly-6G (mouse), clone RB6-8C5, biotin conjugate, Cat. No. MABF1479 is validated for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      CloneRB6-8C5
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ly6g
      Purification MethodPurified by size-exclusion chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted biotin removed from the preparation.

      Flow Cytometry Analysis: 0.06 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF1479 04055977292015