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MABF2754-25UG Anti-IL1F10 (IL-38) Antibody, clone H127C

Anti-IL1F10 (IL-38), clone H127C, Cat. No. MABF2754, is a mouse monoclonal antibody that detects Interleukin-1 family member 10 (IL-38) and is tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

MABF2754-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF2754-25UG
      DescriptionAnti-IL1F10 (IL-38) Antibody, clone H127C
      Alternate Names
      • Interleukin-1 family member 10
      • 1F10
      • Family of interleukin 1-theta
      • FIL1 theta
      • Interleukin-1 HY2
      • IL-1HY2
      • IL-1 theta
      • Interleukin-38
      • IL-38
      Background InformationInterleukin-1 family member 10 (UniProt: Q8WWZ1; also known as IL-1F10, Family of interleukin 1-theta, FIL1 theta, Interleukin-1 HY2, IL-1HY2, Interleukin-1 theta, IL-1 theta, Interleukin-38, IL-38) is encoded by the IL1F10 (also known as FIL1T, IL1HY2, IL38, FKSG75, UNQ6119/PRO20041) gene (Gene ID: 84639) in human. IL-38 is a cytokine with immunomodulatory properties. It is reported to be expressed in fetal skin, spleen and tonsil. Its expression is mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil. It is also shown to be strongly expressed in synovia of rheumatoid arthritis (RA) subjects and is reported to exert anti-inflammatory effects in a murine model of RA. IL-38 alone does not induce cytokine production, but reduces IL-22 and IL-17A production by T cells in response to heat-killed Candida albicans. It also increases the production of IL-6 by dendritic cells stimulated with lipopolysaccharides. IL-38 does not get cleaved by Caspase -1. Clone H160A can be used with H127C (Cat. No. MABF2754B) in sandwich ELISA. (Ref.: Takenaka, S., et al. (2015). Biochem. Biophys. Rep. 4; 386-391; van de Veerdonk, FL., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(8); 3001-3005).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL1F10 (IL-38), clone H127C, Cat. No. MABF2754, is a mouse monoclonal antibody that detects Interleukin-1 family member 10 (IL-38) and is tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesFlow Cytometry Analysis: A representative lot detected IL1F10 (IL-38) in Flow Cytometry applications (Takenaka, S.I., et. al. (2015). Biochem Biophys Rep. 4:386-391).

      Immunohistochemistry Applications: A representative lot detected IL1F10 (IL-38) in Immunohistochemistry applications (Takenaka, S.I., et. al. (2015). Biochem Biophys Rep. 4:386-391).

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected IL1F10 (IL-38) in human skin and human colon cancer tissue sections.

      ELISA Analysis: A representative lot detected IL1F10 (IL-38) in ELISA applications (Takenaka, S.I., et. al. (2015). Biochem Biophys Rep. 4:386-391).

      Western Blotting Analysis: A representative lot detected IL1F10 (IL-38) in Western Blotting applications (Takenaka, S.I., et. al. (2015). Biochem Biophys Rep. 4:386-391).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full-length recombinant human Interleukin-1 family member 10 (IL1F10).
      EpitopeUnknown
      CloneH127C
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone H127C is a mouse monoclonal antibody that detects human IL11F10 (IL-38).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL1F10
      • FIL1T
      • IL1HY2
      • IL38
      • FKSG75
      • UNQ6119/PRO20041
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~18 kDa observed; 16.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human IL1F10 (IL-38) protein.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected IL1F10 (IL-38) recombinant human IL-1F10 (IL-38) protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2754-25UG 04061842625963

      Documentation

      Anti-IL1F10 (IL-38) Antibody, clone H127C FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-IL1F10 (IL-38) Antibody, clone H127C Certificats d'analyse

      TitreNuméro de lot
      Anti-IL1F10 (IL-38), clone H127C - Q3504053 Q3504053

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      IL-38: A new factor in rheumatoid arthritis
      Shin-Ichi Takenaka 1 , Shinjiro Kaieda 1 , Tomotaka Kawayama 1 , Masanobu Matsuoka 1 , Yoichiro Kaku 1 , Takashi Kinoshita 1 , Yuki Sakazaki 1 , Masaki Okamoto 1 , Masaki Tominaga 1 , Katsuya Kanesaki 2 , Asako Chiba 3 , Sachiko Miyake 3 , Hiroaki Ida 1 , Tomoaki Hoshino
      Biochem Biophys Rep  4  386-391  2015

      Afficher le résumé       		29124228 29124228