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MABF3183-25UG Anti-IL-1β Antibody, clone 11G7.3.7

Anti-IL-1β, clone 11G7.3.7, Cat. No. MABF3183, is a mouse monoclonal antibody that detects Interleukin-1β (IL-1β) and is tested for use in Immunohistochemistry (Paraffin) and in Western Blotting.

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MABF3183-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF3183-25UG
      DescriptionAnti-IL-1β Antibody, clone 11G7.3.7
      Alternate Names
      • Interleukin-1 beta
      • Catabolin
      Background InformationInterleukin-1 beta (UniProt: P01584; also known as IL-1 beta, Catabolin) is encoded by the IL1B (also known as IL1F2) gene (Gene ID: 3553) in human. IL-1β is one of the best characterized potent pro-inflammatory cytokines that is critical for host-defense responses to infection and injury. It is produced and secreted by a variety of cell types; however, it is mainly expressed in activated monocytes/macrophages. IL-1β is produced as pro-IL-1β in response to molecular motifs carried by pathogens known as pathogen associated molecular patterns (PAMPs). Its synthesis occurs in two steps and each step is controlled by different stimuli. In the initial step, inflammatory signals, such as lipopolysaccharides, stimulate the synthesis and promote the accumulation of pro-IL-1β in cytoplasm. Following this step pro-IL-1β is cleaved by the caspase-1, the activation of which occurs via recruitment to inflammasome. This step removes the propeptide (1-116) segment. Following caspase-1-dependent processing of pro-IL-1β, mature IL-1β (aa 117-269) is rapidly secreted from cells. IL-1β is known to induce prostaglandin synthesis, neutrophil influx and activation, T-cell and B-cell activation and antibody production. It also promotes Th17 differentiation and synergizes with IL-12 to induce interferon g from T-helper cells. (Ref.: Lopez-Castejon, G., and Brough, D. (2011). Cytokine Growth Factor Rev. 22(4); 189-195; Tominaga, K., et al. (2000). Int. Immunol. 12(2); 151-160).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-1β, clone 11G7.3.7, Cat. No. MABF3183, is a mouse monoclonal antibody that detects Interleukin-1β (IL-1β) and is tested for use in Immunohistochemistry (Paraffin) and in Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-1β in human lung tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length mature recombinant human Interleukin-1β (without the propeptide).
      EpitopeUnknown
      Clone11G7.3.7
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 11G7.3.7 is a mouse monoclonal antibody that detects human Interleukin-1β (IL-1β)
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL1B
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~17 kDa observed; 30.75 kDa calculated for the whole molecule. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human Interleukin-1β (IL-1β).

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected human recombinant Interleukin-1β (IL-1β).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF3183-25UG 04065267613918